1. Weizmannia faecalis sp. nov., isolated from a human stool sample
- Author
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Hoang Thong Kieu, Thi Phuong Thao Pham, Cheikh Ibrahima Lo, Stéphane Alibar, Ludivine Bréchard, Nicholas Armstrong, Philippe Decloquement, Aldiouma Diallo, Cheikh Sokhna, Matthieu Million, Jean-Christophe Lagier, Didier Raoult, Maryam Tidjani Alou, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Vecteurs - Infections tropicales et méditerranéennes (VITROME), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
- Subjects
DNA, Bacterial ,Base Composition ,Culturomics ,MESH: Humans ,[SDV]Life Sciences [q-bio] ,MESH: Bacillaceae ,General Medicine ,Gut microbiota ,Taxono-genomics ,Biochemistry ,Microbiology ,MESH: DNA, Bacterial ,New species ,MESH: RNA, Ribosomal, 16S ,Weizmannia faecalis sp. nov ,MESH: Base Composition ,RNA, Ribosomal, 16S ,Genetics ,Humans ,MESH: Phylogeny ,Molecular Biology ,Bacillaceae ,Phylogeny - Abstract
International audience; Using the culturomics approach, the previously unknown strain Marseille-P8953T, was isolated and classified within the Weizmannia genus. Strain Marseille-P8953T was isolated from the faeces of a healthy subject and consisted of Gram-stain positive, spore-forming, motile rod-shaped cells. A 99.2% similarity was observed between the 16S rRNA gene of strain Marseille-P8953T (accession number LR735539) and that of Weizmannia coagulans strain NBRC 12583T (accession number KX261624), its closest phylogenetic relative, while the genome of strain Marseille-P8953T (3.5 Mpb long, 46.5% GC content) shared the average nucleotide identity by Orthology and digital DNA-DNA Hybridisation values of 95 and 60.4%, respectively. Given the phylogenetic classification and phenotypic characteristics of strain Marseille-P8953T, we propose the creation of a new species within the Weizmannia genus named Weizmannia faecalis (= CSUR P8953T = CECT 9904 T).
- Published
- 2022
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