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31 results on '"Thuret, Gilles"'

1. Histological and in situ microscopic observation of femtosecond laser induced incisions in the crystalline lens

Author: Talbi, Abderazek, Ben Moussa, Olfa, Thuret, Gilles, Gain, Philippe, Sedao, X, Mauclair, Cyril, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), and EA 2521, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (BIIGC), Saint-Etienne, France
Subjects: [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2021

2. Birth and death simulation of human corneal endothelium cells

Author: Thuret, Gilles, He, Zhiguo, Hanielle, Vaitinadapoulle, El Amri, Hatem, Moussa, Olfa Ben, Thomas, Justin, Mascarelli, Frederic, Gain, Philippe, Gavet, Yann, and Lillouch, Fatima
Subjects: [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, endothelial cell density, endothelial homeostasis, human corneal endothelium, [INFO.INFO-MO] Computer Science [cs]/Modeling and Simulation
Abstract: The human corneal endothelium, the most posterior layer of the cornea, is a monolayer of flat cells that are perfectly joined and are essential for maintaining its transparency over time. Long considered to be non-regenerative in adults, several recent studies have nevertheless shown that some cells can proliferate in the periphery of the endothelium. At birth, the endothelial cell density (ECD, number of cells per unit area) is very high and theendothelial mosaic consists of perfect hexagons. During life, some cells disappear, others divide and the mosaic reorganizes itself: its polymorphism and polymegethism increase. Thus, after 20 years, the central WFD decreases by 0.6% per year, remaining higher in the periphery. However, endothelial homeostasis remains poorly understood, in particular how new peripheral cells partially compensate for the disappearance of other more central cells. The cells form a regular pavement, which can be considered mathematically as a Voronoi pavement without holes. We simulated the organization and reorganization of the cells by means of a centered Voronoi diagram, which was created iteratively by moving the seeds of the diagram to the geometric center of the cells. The initialization of the seeds was done with a uniform distribution, and a few centering iterations were sufficient to have cells close to hexagons. Subsequently, we simulated the evolution through cycles of cell death/birth, followed by cell centering. This new simulation tool will allow us to test many hypotheses on the number and frequency of cellular events (mitosis and death) in order to elucidate endothelial pathophysiology.
Published: 2021

3. 4812 - A0028 Gangliosides in the retina: a comparative inventory with brain and other ocular structures

Author: Masson, Elodie, Sibille, Estelle, Martine, Lucy, Berdeaux, Olivier, Bron, Alain, Creuzot Garcher, Catherine, He, Zhiguo, Thuret, Gilles, Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Department of Ophtamology, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Laboratory 'Biology, Imaging, and Engineering of Corneal Grafts' EA2521, Université Jean Monnet (Saint-Etienne), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), ProdInra, Archive Ouverte, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon )
Subjects: retina, ganglioside, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, brain, eye diseases, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, lc/ms, sense organs, human, ceramide, sphingolipid, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, plasma, mass spectrometry
Abstract: Purpose: Gangliosides (GG) make an extremely diverse family of glycosphingolipids particularly abundant in the brain and neural tissues. While it is known that alterations in GG metabolism are associated with visual defects, the precise biological roles of these compounds in the retina are still largely unknown. In this context, we performed an exhaustive inventory and thorough characterization of the human eye GG aiming to reveal specificities and common features of the retina compared to other ocular tissues and brain.Methods: Retina, RPE/Choroid, ciliary body, optic nerve and brain samples were collected from deceased human donors (N=7). GG were extracted and analysed by a comprehensive approach relying on various techniques. First, GG content and class composition were assessed by high-performance thin layer chromatography followed by colorimetric revelation. Second, identification and relative quantification of the different ceramide types represented in each GG class were achieved using liquid chromatography coupled to mass spectrometry. Finally, high-resolution mass spectrometry allowed for the identification of original GG classes and structural characterization of the ceramide moieties (sphingoïd base and fatty acid).Results: Retina contained the highest amount of GG among the ocular tissues tested, although much less than brain. It also presented with a wide diversity of GG classes. As other types of nervous tissues, the retina contained high levels of tetraosylGG but the prominence of GD3, GT3 and acetylated GT3 appeared as a specificity of this tissue. Retina also exhibited the largest diversity of ceramide types sharing common features with brain. They both had especially high proportions of monounsaturated ceramides and consecutively low proportions of polyunsaturated ceramides, as well as low proportions of ceramides shorter than 36 carbons or longer than 41 carbons. Moreover, GG of the retina contained 4 different types of sphingoïd bases: mostly d18:1 as every other tissues but also significant levels of d20:1 as the brain and optic nerve and in minor proportions d16:1 and d18:2 also found in ciliary body.
Published: 2016

4. Gangliosides in the retina: a comparative inventory with brain and other ocular structures

Author: Masson, Elodie A.Y., Sibille, Estelle, Martine, Lucy, Berdeaux, Olivier, Bron, Alain Marie, Creuzot Garcher, Catherine P., He, Zhiguo, Thuret, Gilles, Brétillon, Lionel, OEil, nutrition et signalisation cellulaire [CSGA], Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Mécanismes de la perception et plasticité chimio-sensorielle [CSGA], Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), National Institute for Research on Agronomy (INRA) Regional Council of Burgundy European Regional Development Fund (FEDER), ARVO, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Biologie, Ingénierie et Imagerie de la Greffe de Cornée ( EA 2521, JE2521, IFR143 ), Université Jean Monnet [Saint-Étienne] ( UJM ), and université de Bourgogne, CSGA
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, Retina diseases, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience
Published: 2016

5. Delivery of macromolecules into the endothelium of whole ex vivo human cornea by femtosecond laser-activated carbon nanoparticles

Author: Jumelle, Clothile, Mauclair, Cyril, Houzet, Julien, Bernard, Aurelien, He, Zhiguo, Forest, Fabien, Perrache, Ch., Gain, Philippe, Thuret, Gilles, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Service d'Anatomie et Cytologie Pathologiques, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Institut Universitaire de France (IUF), and Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
Subjects: [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2016

6. Associations between erythrocyte and ciliary epithelium phosphatidylcholine concentrations in healthy humans

Author: Acar, Niyazi, Berdeaux, Olivier, Zhiguo, H.E., Cabaret, Stéphanie, Gain, Philippe, Creuzot Garcher, Catherine, Thuret, Gilles, Bron, Alain, Brétillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université Jean Monnet (Saint-Etienne), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Archive Ouverte
Subjects: [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: absent
Published: 2015

7. Longitudinal study of retinal status using optical coherence tomography after acute post-cataract endophthalmitis

Author: Zhou, Thierry, Creuzot Garcher, Catherine P., Thuret, Gilles, Bron, Alain M., Aptel, Florent, Cornut, Pierre Loïc, Chiquet, Christophe, Centre Hospitalier Universitaire de Grenoble, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), CHU St Etienne, CHU de Grenoble , Centre Hospitalier Universitaire de Grenoble, France, Clinique du Val d'Ouest, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), La Clinique du Val d'Ouest, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and ProdInra, Archive Ouverte
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: absent
Published: 2015

8. Delivery of molecules into human corneal endothelial cells by carbon nanoparticles activated by femtosecond laser

Author: Jumelle, Clothile, Mauclair, Cyril, Houzet, Julien, Bernard, Aurelien, He, Zhiguo, Forest, Fabien, Peoc'H, Michel, Acquart, Sophie, Gain, Philippe, Thuret, Gilles, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Laboratoire Hubert Curien [Saint Etienne] (LHC), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Service d'Anatomie et Cytologie Pathologiques, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Department of Pathology, Saint Etienne University Hospital, Etablissement Français du Sang (EFS), EFS, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), and Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

9. Dietary fatty acids and the prevention of Age-related Macular Degeneration: retinal incorporation and beyond

Author: Brétillon, Lionel, Acar, Niyazi, He, Zhiguo, Grégoire, Stéphane, Bron, Alain, Thuret, Gilles, Creuzot Garcher, Catherine, Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet [Saint-Étienne] (UJM), Ophthalmology, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), and ProdInra, Migration
Subjects: [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, retina, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, aging, fatty acid, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience
Published: 2014

10. Femtosecond laser activated carbon nanoparticles for corneal gene therapy

Author: Jumelle, Clothile, Bernard, Aurélien, Mauclair, Cyril, Campolmi, N., Granier, J., Egaud, Gregory, Sabido, Odile, Gain, Philippe, Thuret, Gilles, and Mauclair, Cyril
Subjects: [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, sense organs
Abstract: Cell permeabilization allows us to deliver all sorts of molecules, especially genetic material able to modify cell behavior, into cells. A new cell therapy method published in Nature Nanotechnology in 2011 [1] consists in ephemerally permeabilizing cell membranes using a photo-acoustic reaction produced by carbon nanoparticles and femtosecond laser. Authors introduced a small molecule called calcein into cells in suspension whose membrane is normally impermeable with a good efficiency (until 90%) and a very low toxicity (
Published: 2014

11. Effect of an adjunctive first-line intravitreal betamethasone injection in postoperative endophthalmitis

Author: Koehrer, Philippe, Chiquet, Christophe, Thuret, Gilles, Delbosc, Bernard Y., Bourcier, Tristan, Berrod, Jean-Paul, Bron, Alain M., Garcher, Catherine P., Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Grenoble, CHU St-Etienne, Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Centre Hospitalier Universitaire de Strasbourg (CHU de Strasbourg ), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, 445 cataract, 487 corticosteroids, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, 513 endophthalmitis, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience
Published: 2014

12. Circulating markers of retinal and optic nerve lipids

Author: Acar, Niyazi, Berdeaux, Olivier, He, Zhiguo, Cabaret, Stéphanie, Gain, Philippe, Thuret, Gilles, Creuzot Garcher, Catherine, Bron, Alain, Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), EA2521, Université Jean Monnet (Saint-Etienne), Department of Ophthalmology, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)
Subjects: [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, clinical trial, cell, 583 lipids, eye diseases, essai clinique, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, test de laboratoire, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, lipid, 467 clinical laboratory testing, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, lipids (amino acids, peptides, and proteins), degénération rétinienne, cellule, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 695 retinal degenerations: cell biology, lipide
Abstract: Purpose Blood lipids are frequently used as a surrogate of lipid composition of peripheral tissues. Even if it is well accepted, such a relationship has never been clearly demonstrated for the eye. The aim of this study was to determine in human samples whether a lipidomic approach based on red blood cells could reveal associations between circulating and ocular lipid profiles. Methods Red blood cells, retinas and optic nerves were collected from 9 human donors. The lipidomic analyses on these tissues were done by both, gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS/MS). Results Gas chromatographic approach did not show any relevant association between circulating and ocular lipids except for arachidonic acid whose circulating amounts were positively associated with its retinal and optic nerve levels. However, significant associations emerged from LC-ESI-MS/MS analyses. Indeed, phospholipid species in red blood cells were positively or negatively associated with representative pools of retinal DHA (docosahexaenoic acid), retinal VLC-PUFA (very-long chain polyunsaturated fatty acids) or optic nerve plasmalogens. Conclusions First, our results show that LC-ESI-MS/MS methodology is more appropriate than gas chromatography for lipidomics on red blood cells, and further extrapolation to ocular lipids. Second, this study has identified several individual lipid species that can be good candidates to represent circulating biomarkers of ocular lipids.
Published: 2013

13. Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages.: DLL4's opposing effects in choroidal neovascularization

Author: Camelo, Serge, Raoul, William, Lavalette, Sophie, Calippe, Bertrand, Cristofaro, Brunella, Levy, Olivier, Houssier, Marianne, Sulpice, Eric, Jonet, Laurent, Klein, Christophe, Devevre, Estelle, Thuret, Gilles, Duarte, Antonio, Eichmann, Anne, Leconte, Laurence, Guillonneau, Xavier, Sennlaub, Florian, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Faculté de Médecine, CHU Saint-Etienne-Université Jean Monnet - Saint-Étienne (UJM)-Assistance Publique des Hôpitaux de St Etienne, Instituto Gulbenkian de Ciência [Oeiras] (IGC), Fundação Calouste Gulbenkian, Faculdade de Medicina Veterinária (CIISA), Centre de Recherche Cardiovasculaire de Lariboisiere, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Ophtalmologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôtel-Dieu, ANR blanc AO5120DD ANR Genopat R09099DS ERC-2007 Starting Grant 210345, and Raoul, William
Subjects: MESH: DNA Primers, Notch, genetic structures, age related macular degeneration, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, DLL4, MESH: Base Sequence, MESH: Mice, Inbred C57BL, MESH: Reverse Transcriptase Polymerase Chain Reaction, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Blotting, Western, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, MESH: Intercellular Signaling Peptides and Proteins, MESH: Mice, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, MESH: Choroidal Neovascularization, eye, eye diseases, macrophages, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, sense organs, Angiogenesis, MESH: Endothelium, Vascular, MESH: Macrophages, Peritoneal
Abstract: International audience; Inflammatory neovascularization, such as choroidal neovascularization (CNV), occur in the presence of Notch expressing macrophages. DLL4s anti-angiogenic effect on endothelial cells (EC) has been widely recognized, but its influence on Notch signaling on macrophages and its overall effect in inflammatory neovascularization is not well understood. We identified macrophages and ECs as the main Notch 1 and Notch 4 expressing cells in CNV. A soluble fraction spanning Ser28-Pro525 of the murine extracellular DLL4 domain (sDLL4/28-525) activated the Notch pathway, as it induces Notch target genes in macrophages and ECs and inhibited EC proliferation and vascular sprouting in aortic rings. In contrast, sDLL4/28-525 increased pro-angiogenic VEGF, and IL-1β expression in macrophages responsible for increased vascular sprouting observed in aortic rings incubated in conditioned media from sDLL4/28-525 stimulated macrophages. In vivo, Dll4(+/-) mice developed significantly more CNV and sDLL4/28-525 injections inhibited CNV in Dll4(+/-) CD1 mice. Similarly, sDLL4/28-525 inhibited CNV in C57Bl6 and its effect was reversed by a γ-secretase inhibitor that blocks Notch signaling. The inhibition occurred despite increased VEGF, IL-1β expression in infiltrating inflammatory macrophages in sDLL4/28-525 treated mice and might be due to direct inhibition of EC proliferation in laser-induced CNV as demonstrated by EdU labelling in vivo. In conclusion, Notch activation on macrophages and ECs leads to opposing effects in inflammatory neovascularization in situations such as CNV.
Published: 2012

14. Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages. : DLL4's opposing effects in choroidal neovascularization

Author: Camelo, Serge, Raoul, William, Lavalette, Sophie, Calippe, Bertrand, Cristofaro, Brunella, Levy, Olivier, Houssier, Marianne, Sulpice, Eric, Jonet, Laurent, Klein, Christophe, Devevre, Estelle, Thuret, Gilles, Duarte, Antonio, Eichmann, Anne, Leconte, Laurence, Guillonneau, Xavier, Sennlaub, Florian, Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de la Vision, Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ), Centre interdisciplinaire de recherche en biologie ( CIRB ), Collège de France ( CdF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Biologie à Grande Échelle ( BGE - UMR S1038 ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Grenoble Alpes [Saint Martin d'Hères]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Faculté de Médecine, CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] ( UJM ) -Assistance Publique des Hôpitaux de St Etienne, Instituto Gulbenkian de Ciência [Oeiras] ( IGC ), Fundação Calouste Gulbenkian, Faculdade de Medicina Veterinária ( CIISA ), Universidade Técnica de Lisboa, Centre de Recherche Cardiovasculaire de Lariboisiere, Université Paris Diderot - Paris 7 ( UPD7 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Département d'Ophtalmologie, Assistance publique - Hôpitaux de Paris (AP-HP)-Hôtel-Dieu, ANR blanc AO5120DD ANR Genopat R09099DS ERC-2007 Starting Grant 210345, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] (UJM)-Assistance Publique des Hôpitaux de St Etienne, Instituto Gulbenkian de Ciência [Oeiras] (IGC), Faculdade de Medicina Veterinária (CIISA), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôtel-Dieu, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Faculté de Médecine Jacques Lisfranc [Université Saint-Etienne], and Université Jean Monnet - Saint-Étienne (UJM)
Subjects: MESH: DNA Primers, Notch, genetic structures, age related macular degeneration, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH : Blotting, Western, MESH : Mice, Inbred C57BL, MESH: Base Sequence, DLL4, MESH: Mice, Inbred C57BL, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH : Mice, MESH: Blotting, Western, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, MESH: Intercellular Signaling Peptides and Proteins, MESH: Mice, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH : Intercellular Signaling Peptides and Proteins, MESH : Choroidal Neovascularization, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, MESH: Choroidal Neovascularization, MESH : Reverse Transcriptase Polymerase Chain Reaction, eye, eye diseases, macrophages, MESH : Endothelium, Vascular, MESH : Macrophages, Peritoneal, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, MESH : Base Sequence, MESH : DNA Primers, MESH: Endothelium, Vascular, sense organs, Angiogenesis, MESH : Animals, MESH: Macrophages, Peritoneal
Abstract: International audience; Inflammatory neovascularization, such as choroidal neovascularization (CNV), occur in the presence of Notch expressing macrophages. DLL4s anti-angiogenic effect on endothelial cells (EC) has been widely recognized, but its influence on Notch signaling on macrophages and its overall effect in inflammatory neovascularization is not well understood. We identified macrophages and ECs as the main Notch 1 and Notch 4 expressing cells in CNV. A soluble fraction spanning Ser28-Pro525 of the murine extracellular DLL4 domain (sDLL4/28-525) activated the Notch pathway, as it induces Notch target genes in macrophages and ECs and inhibited EC proliferation and vascular sprouting in aortic rings. In contrast, sDLL4/28-525 increased pro-angiogenic VEGF, and IL-1β expression in macrophages responsible for increased vascular sprouting observed in aortic rings incubated in conditioned media from sDLL4/28-525 stimulated macrophages. In vivo, Dll4(+/-) mice developed significantly more CNV and sDLL4/28-525 injections inhibited CNV in Dll4(+/-) CD1 mice. Similarly, sDLL4/28-525 inhibited CNV in C57Bl6 and its effect was reversed by a γ-secretase inhibitor that blocks Notch signaling. The inhibition occurred despite increased VEGF, IL-1β expression in infiltrating inflammatory macrophages in sDLL4/28-525 treated mice and might be due to direct inhibition of EC proliferation in laser-induced CNV as demonstrated by EdU labelling in vivo. In conclusion, Notch activation on macrophages and ECs leads to opposing effects in inflammatory neovascularization in situations such as CNV.
Published: 2012

15. Relationship between baseline clinical data and microbiologic spectrum in 100 patients with acute postcataract endophthalmitis

Author: Cornut, Pierre-Loïc, Thuret, Gilles, Creuzot-Garcher, Catherine, Maurin, Max, Pechinot, Andre, Bron, Alain, Gain, Philippe, Carricajo, Anne, Denis, Philippe, Romanet, Jean-Paul, Vandenesch, Francois, Chiquet, Christophe, Renseigné, Non, Department of Ophtalmology, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Laboratoire de Microbiologie Médicale et Moléculaire, Université de Bourgogne (UB), Department of Microbiology, CHU Saint-Etienne, Service de physiologie digestive, urinaire, respiratoire et de l'exercice [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Hôpital Charles Nicolle [Rouen], Centre National de référence des Staphylocoques, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL), Université Joseph Fourier - Grenoble 1 (UJF), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Service de physiologie digestive, urinaire, respiratoire et de l'exercice [Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU), Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), Biologie, Ingénierie et Imagerie de la Greffe de Cornée ( EA 2521, JE2521, IFR143 ), Université Jean Monnet [Saint-Étienne] ( UJM ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble, Université de Bourgogne ( UB ), Hôpital Charles Nicolle [Rouen]-CHU Rouen-Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Hospices Civils de Lyon ( HCL ), Université Joseph Fourier - Grenoble 1 ( UJF ), and Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN)
Subjects: Intraocular pressure, Visual acuity, genetic structures, medicine.medical_treatment, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, MESH : Aged, MESH : Prospective Studies, MESH : Analysis of Variance, Fundus (eye), MESH: Visual Acuity, 0302 clinical medicine, Endophthalmitis, MESH: Aged, 80 and over, MESH : Female, MESH : Intraocular Pressure, MESH: Aged, 0303 health sciences, MESH : Visual Acuity, MESH: Cataract Extraction, MESH: Middle Aged, biology, MESH : Acute Disease, MESH: Staphylococcus, General Medicine, baseline clinical data, 3. Good health, MESH: Acute Disease, medicine.symptom, MESH : Visual Perception, medicine.medical_specialty, MESH : Cataract Extraction, MESH : Male, MESH : Endophthalmitis, MESH: Streptococcus, Staphylococcus lugdunensis, Hypopyon, acute postcataract endophthalmitis, 03 medical and health sciences, postoperative endophthalmitis, MESH: Intraocular Pressure, MESH : Streptococcus, Ophthalmology, MESH: Analysis of Variance, medicine, microbiologic spectrum, MESH: Endophthalmitis, Medical history, MESH : Middle Aged, MESH : Bacteria, MESH : Aged, 80 and over, 030304 developmental biology, MESH : Staphylococcus, MESH: Humans, MESH: Visual Perception, business.industry, MESH : Humans, Phacoemulsification, biology.organism_classification, medicine.disease, MESH: Male, MESH: Prospective Studies, MESH: Bacteria, 030221 ophthalmology & optometry, business, MESH: Female, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: EA MERS CT3 Enjeu 3; International audience; PURPOSE: To correlate the initial ocular presentation with bacterial identification in 100 patients with acute postcataract endophthalmitis. METHODS: This was a prospective multicenter study. Demographic data, medical history, and the initial eye examination data were recorded on a standardized form. The relationship between bacterial identification and clinical factors at baseline was studied using univariate and multivariate analyses. RESULTS: One hundred patients were admitted to the hospital with a median delay of 6 days after cataract surgery. The main symptoms were loss of vision (94.9%) and pain (75.5%). Major clinical signs were hypopyon (72%), pupillary fibrin membrane (77.5%), and loss of fundus visibility (90%). Baseline factors significantly associated with microbiologic identification were as follows: diabetes mellitus, a shorter delay of onset, initial visual acuity limited to light perception, higher intraocular pressure, chemosis, pupillary fibrin membrane, loss of the red reflex, and reduced fundus visibility. As compared with other bacteria, the identification of Streptococcus species (n = 19) was more frequently associated with male gender, diabetes mellitus, initial visual acuity limited to light perception, and pain. The Staphylococcus aureus and Staphylococcus lugdunensis group (n = 14) differed from other coagulase-negative Staphylococcus groups (n = 33) in that those patients had greater hypopyon height. CONCLUSION: The baseline features of acute endophthalmitis after cataract surgery in the era of phacoemulsification are similar to those reported in the Endophthalmitis Vitrectomy Study 15 years ago and differ according to the bacterial species. The association between the clinical signs and the microbiologic identification suggests that initial characteristics other than visual acuity may be useful in identifying patients presumed to be infected with a virulent species.
Published: 2012

16. Study of stromal femtosecond laser ablation for deep corneal cut optimization

Author: Bernard, Aurélien, Audouard, Eric, Thuret, Gilles, Peoc'H, Michel, Dumollard, Jean-Marc, Granier, Julien, Soder, Hervé, Gain, Philippe, and Mauclair, Cyril
Subjects: genetic structures, [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, sense organs, eye diseases
Abstract: Femtosecond laser has become a common tool in corneal surgery during the last years. The first clinical application of femtosecond lasers (FL) in ophthalmology was in refractive surgery (LASIK : laser in situ keratomileusis) for the preparation of cornea flaps [1]. Recently, FL applications have been extend to penetrating and lamellar keratoplasty. However, commercially available femtosecond laser in ophthalmology have already shown limitations in these new applications, in particular in oedematous cornea (pathologic or stored by eye banks). Experimental and clinical investigations of deep lamellar and penetrating graft in oedematous corneas have often shown bad quality dissections, with the presence of numerous remaining tissue bridges [2, 3]. In order to improve the results of these dissections, a fine knowledge of the dissected biological material is essential. The human cornea is a complex tissue made of different layers. The stroma, central layer mainly made of collagen fibrils and water, represent more than 90% of the whole corneal thickness. Collagen fibrils organization and hydration in the stroma is not homogeneous too. Anterior stroma owns a complex collagen fiber branching patterns, which decreased logarithmically in posterior direction [4].The purpose of this study is to investigate interactions between FL and human corneal stroma. The study has been realised on human corneal grafts unsuitable for transplantation. Corneal grafts have been prepared with a mechanical corneal microkeratome. A lamellar cut from anterior side has been performed in at two different depths: 50µm for anterior stroma study and 350µm for posterior stroma study (see figure 1-c). This preparation is intended to prevent any interference between the incident laser and the other corneal layers. Ablation thresholds and ablation rates have been studied on remaining anterior and posterior corneal stroma for different processing configuration, such as laser fluence and pulse number. Results of this study will show if the inhomogeneous histological organization of the corneal stroma and pulse number have implications on ablation thresholds and rates. If this is the case, these results could be used to improve FL ablation parameters for deep cornea cut.
Published: 2012

17. Decreasing the dietary intake of linoleic acid promotes the efficacy of dietary long chain omega-3 fatty acids to incorporate the rat retina and modifies gene expression

Author: Simon, Emilie, Bardet, Bertrand, Grégoire, Stéphane, Thuret, Gilles, Acar, Niyazi, Bron, Alain, Gain, Philippe, Creuzot Garcher, Catherine, Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Ophtalmologie, Université Jean Monnet (Saint-Etienne), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), ProdInra, Archive Ouverte, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon )
Subjects: [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, genetic structures, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, eye diseases
Abstract: Decreasing the dietary intake of linoleic acid promotes the efficacy of dietary long chain omega-3 fatty acids to incorporate the rat retina and modifies gene expression. annual meeting of the association-for-research-in-vision-and-ophthalmology (ARVO)
Published: 2011

18. Friends group: Post trauma endophthalmitis

Author: Cornut, Pierre-Loïc, Elbichara, Youssef, Vandenesch, François, Burillon, Carole, Romanet, Jean-Paul, Bron, Alain M., Thuret, Gilles, Boisset, Sandrine, Maurin, Max, Chiquet, Christophe, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Centre Hospitalier Universitaire de Grenoble, Centre National de Référence des Staphylocoques, Centre Hospitalier Universitaire de Lyon, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre Hospitalier Universitaire de Saint-Etienne, Université Joseph Fourier - Grenoble 1 (UJF), Hospices Civils de Lyon ( HCL ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Université Joseph Fourier (Grenoble 1) ( UJF ), and ProdInra, Archive Ouverte
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, genetic structures, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.OT ] Life Sciences [q-bio]/Other [q-bio.OT], [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, eye diseases, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Friends group: Post trauma endophthalmitis. annual meeting of the association-for-research-in-vision-and-ophthalmology (ARVO)
Published: 2011

19. Mesure d'épaisseur de lentilles de contact et détection de traces de chirurgie réfractive dans des cornées par interférométrie à faible longueur de cohérence

Author: Verrier, Isabelle, Veillas, Colette, Lépine, Thierry, Thuret, Gilles, Gain, Philippe, and Verrier, Isabelle
Subjects: [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Published: 2011

20. Quantitative analysis of fatty acids on choline- and ethanolamine-phospholipids in human ocular and non-ocular tissues by liquid chromatography/tandem mass spectrometry (LC-ESI-MS/MS)

Author: Berdeaux, Olivier, Cabaret, Stéphanie, Martine, Lucy, Thuret, Gilles, Grégoire, Stéphane, Gain, Philippe, Creuzot Garcher, Catherine P., Bron, Alain M., Acar, Niyazi, Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Faculty of Medicine, Université Jean Monnet (Saint-Etienne), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), and ProdInra, Archive Ouverte
Subjects: [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, genetic structures, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, lipids (amino acids, peptides, and proteins), [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, eye diseases
Abstract: Quantitative analysis of fatty acids on choline- and ethanolamine-phospholipids in human ocular and non-ocular tissues by liquid chromatography/tandem mass spectrometry (LC-ESI-MS/MS). annual meeting of the association-for-research-in-vision-and-ophthalmology (ARVO)
Published: 2011

21. Factors of good visual outcome in acute post-cataract endophthalmitis (friends group)

Author: Combey de Lambert, Aurélie, Thuret, Gilles, Cornut, Pierre Loïc, Bron, Alain, Maurin, Max, Creuzot Garcher, Catherine, Vandenesch, François, Denis, Philippe, Romanet, Jean-Paul, Chiquet, Christophe, ProdInra, Migration, CHU Grenoble, CHU St-Etienne, Centre Hospitalier Universitaire de Lyon, Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, genetic structures, endophthalmitis, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, clinical (human) or epidemiologic studies: biostatistics/epidemiology methodology, perception, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, eye diseases
Abstract: International audience; Purpose: To analyze initial clinical factors of good visual outcome in patients with acute endophthalmitis following cataract surgery. Methods: A prospective study was performed in 4 University hospital (Dijon, Grenoble, Lyon, Saint-Etienne, FRIENDS group) on 100 patients with acute post-cataract endophthalmitis. Factors related to cataract surgery (complications), the initial presentation and the microbiological identification were analyzed according to the final visual outcome using univariate and multivariate analysis (SPSS 12.0). Results: 46% out of the patients had a final visual acuity less than or equal to 0.3 logMar (good visual outcome) at 6 months while 10% had light perceptions. Patients with good visual outcome differed from the patients with poor final visual acuity for the duration of cataract surgery (p=0.05), initial visual acuity (> LP, p=0.005), the visibility of fundus (p=0.04) and the identification of a coagulase negative Staphylococcus (p=0.05). On the other hand, patients with a poor visual outcome (> 0.3 LogMAR) were older (p=0.004), had more frequently cornea oedema (p=0.05) and a larger hypopion (p=0.01) at admission, more frequently complications at the time of cataract surgery (p=0.005), and a bacterium was more frequently identified (p=0.005). Multivariate analysis showed that age, complications at the time of cataract surgery and identification of bacteria were independent factors of visual prognosis. Conclusions: Factors of visual outcome in acute postcataract endophthalmitis identified in this prospective study (2004-2007) were similar to those reported by the EVS 15 years ago. Given the bias associated with the therapeutic strategy, pars plana vitrectomy is associated with predictive factors of poor visual outcome. Identificaton of these predictive factors at presentation should allow a better identification of patients needed an aggressive treatment.
Published: 2010

22. Analysis of diluted vitreous samples from vitrectomy is useful in eyes with severe acute postoperative endophthalmitis

Author: Chiquet , Christophe, Maurin , Max, Thuret , Gilles, Benito , Yvonne, Cornut , Pierre-Loïc, Creuzot-Garcher , Catherine, Rouberol , Frédéric, Pechinot , André, Lina , Gérard, Romanet , Jean-Paul, Bron , Alain, Vandenesch , François, Renseigné , Non, Department of Ophtalmology, CHU Grenoble, Département des agents infectieux, Service d'ophtalmologie, Université Jean Monnet [Saint-Étienne] ( UJM ) -Hôpital Bellevue, Immunité infection vaccination ( I2V ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Bactériologie, Hospices Civils de Lyon ( HCL ), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Laboratoire de Microbiologie Médicale et Moléculaire, Université de Bourgogne ( UB ), Université Jean Monnet [Saint-Étienne] (UJM)-Hôpital Bellevue, Immunité infection vaccination (I2V), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR128-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), and Université de Bourgogne (UB)
Subjects: Male, genetic structures, medicine.medical_treatment, Colony Count, Microbial, MESH : Aged, MESH : Prospective Studies, MESH : Vitrectomy, Vitrectomy, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Polymerase Chain Reaction, Eye Infections, Bacterial, Cohort Studies, Postoperative Complications, 0302 clinical medicine, Endophthalmitis, RNA, Ribosomal, 16S, MESH: Postoperative Complications, MESH : DNA, Ribosomal, MESH : DNA, Bacterial, MESH : Female, Prospective Studies, MESH: Cohort Studies, MESH : Polymerase Chain Reaction, MESH: Aged, MESH: Cataract Extraction, 0303 health sciences, MESH: DNA, Ribosomal, MESH: Eye Infections, Bacterial, MESH : Acute Disease, Outcome measures, Diagnostic test, Bacterial Typing Techniques, 3. Good health, MESH: RNA, Ribosomal, 16S, medicine.anatomical_structure, Acute Disease, MESH: Acute Disease, MESH : Eye Infections, Bacterial, Female, DNA, Bacterial, Pars plana, medicine.medical_specialty, MESH : Cataract Extraction, MESH : Colony Count, Microbial, MESH : Male, MESH : Endophthalmitis, MESH : Cohort Studies, Cataract Extraction, DNA, Ribosomal, MESH: Bacterial Typing Techniques, 03 medical and health sciences, McNemar's test, Ophthalmology, medicine, Humans, MESH: Endophthalmitis, MESH : Bacteria, MESH: Colony Count, Microbial, Aged, Brain-heart Infusion broth, MESH: Humans, Bacteria, 030306 microbiology, business.industry, MESH: Vitreous Body, MESH : Humans, MESH: Polymerase Chain Reaction, medicine.disease, MESH: DNA, Bacterial, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, MESH: Prospective Studies, eye diseases, Vitreous Body, MESH : RNA, Ribosomal, 16S, MESH: Bacteria, MESH: Vitrectomy, 030221 ophthalmology & optometry, MESH : Bacterial Typing Techniques, MESH : Postoperative Complications, business, MESH : Vitreous Body, MESH: Female, Acute postoperative endophthalmitis
Abstract: Purpose This study was designed to compare the diagnostic yield of microbiological analysis performed on diluted and undiluted vitreous samples from pars plana vitrectomy (PPV) in patients with acute postcataract endophthalmitis. Design Cohort study, evaluation of diagnostic test or technology. Participants Patients with acute postcataract endophthalmitis ( Methods Undiluted and diluted vitreous samples were taken from 34 consecutive patients at the beginning of PPV as part of the multicenter prospective study of the French Institutional Endophthalmitis Study (FRIENDS) group. Vitrectomy was performed after 1 (n = 12) or 2 (n = 22) intravitreous antibiotic injections. McNemar's nonparametric test was used to compare culture and polymerase chain reaction (PCR) results between diluted and undiluted samples. Main Outcome Measures Rate of positivity of conventional culture (brain heart infusion broth) and eubacterial PCR tests from undiluted and diluted vitreous samples. Results The microbiological analysis of both undiluted and diluted vitreous samples detected and identified a bacterial pathogen in 26 out of 34 cases (76.4%). The analysis of undiluted and diluted vitreous at the time of PPV, using eubacterial PCR and conventional culture, gave similar results ( P = 0.99; McNemar test). However, eubacterial PCR was more sensitive than culture in detecting bacteria in vitreous at the time of PPV (76% vs 6%; P = 0.001; McNemar test). The difference in sensitivity between the 2 techniques was primarily associated with false-negative culture results for undiluted samples (2/3 of cases), mainly for coagulase-negative staphylococci. Conclusions The microbiological results obtained combining PCR and culture techniques were similar for diluted vitreous and undiluted vitreous analysis. When eubacterial PCR is available, sampling diluted vitreous, an easier procedure, may replace sampling undiluted vitreous. Financial Disclosure(s) The authors have no proprietary or commercial interest in any of the materials discussed in this article.
Published: 2009

23. A new HPLC-ESI-MS/MS method to characterize and quantify phosphatidyl-choline with VLC-PUFA: Application to human retina

Author: Berdeaux, Olivier, Martine, Lucy, Cabaret, Stéphanie, Thuret, Gilles, Grégoire, Stéphane, Gain, Philippe, Creuzot Garcher, Catherine, Bron, Alain, Acar, Niyazi, Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Biology, Engineering and Imaging of Corneal Grafts, University Jean Monnet, Centre Hospitalier Universitaire de Saint-Etienne, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Biologie, Ingénierie et Imagerie de la Greffe de Cornée ( EA 2521, JE2521, IFR143 ), Université Jean Monnet [Saint-Étienne] ( UJM ), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), and Labo/service de l'auteur, Ville service, Pays service.
Subjects: retina, [ SDV ] Life Sciences [q-bio], lipid, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [SDV]Life Sciences [q-bio], lipids (amino acids, peptides, and proteins), sense organs, clinical laboratory testing, eye diseases
Abstract: Purpose: Mutations in the ELOVL4 gene have been found in Stargardt-like macular dystrophy or STD3. Previous studies have shown that ELOVL4 is involved in the biosynthesis of very long chain polyunsaturated fatty acids (VLC-PUFA). The aim of this work was to develop a HPLC-ESI-MS/MS method of characterization and quantification of dipolyunsaturated phosphatidyl-choline (PC) molecular species containing VLC-PUFA and to apply it on retinas from human donors. Methods: Eyeballs were collected from calf as well as from nine human donors (body donation to Science). The neural retina was dissected from the RPE/choroid. Following lipid extraction, phosphorus content of total phospholipids was determined.Using a triple quadrupole MS instrument, PC molecular species were structurally characterized by collision-induced dissociation in the negative mode with a method based on normal-HPLC-ESIMS/MS. PC molecular species were then quantified using precursor ion scanning of m/z 184amu in the positive mode. Results: The characterization of PC species was done on bovine retinas. Among them, 28 were dipolyunsaturated PC species containing one VLC-PUFA (C24 to C36) with three to six double bonds. VLC-PUFA were always in the sn-1 position whilst PUFA at the sn-2 position was exclusively docosahexaenoic acid (DHA, C22:6.n-3). Most of these VLC-PUFA-containing dipolyunsaturated PC were detected and quantified in human retinas. The main represented compounds were those having VLC-PUFA of 32 carbon atoms (C32:3, C32:4, C32:5 and C32:6) and 34 carbon atoms (C34:3, C34:4, C34:5 and C34:6). Dipolyunsaturated PC with 36:5 and 36:6 were detected in lower quantities. Conclusions: This new HPLC-ESI-MS/MS method is sensitive and specific enough to structurally characterize and quantify all molecular species of PC, including those esterified with VLC-PUFA. This technique is valuable for a precise characterization of PC containingVLC-PUFA in retina and may be useful for better understanding their implication in the pathogenesis of STD3.
Published: 2009

24. Would circulating cholesteryl esters be considered as markers for fatty acid composition of the human retina?

Author: Brétillon, Lionel, Acar, Niyazi, Grégoire, Stéphane, Thuret, Gilles, Bron, Alain, Gain, Philippe, Creuzot-Garcher, Catherine, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Biology, Engineering and Imaging of Corneal Grafts, Centre Hospitalier Universitaire de Saint-Etienne, Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), and Labo/service de l'auteur, Ville service.
Subjects: lipid, nutritional factor, retina, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, lipids (amino acids, peptides, and proteins), sense organs, [ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences, eye diseases
Abstract: Purpose: Lecithin Cholesterol Acyl Transferase (LCAT) and Cholesteryl Ester Transfer Protein (CETP) are the main lipid transfer proteins which activities are involved in the metabolism of cholesteryl esters (CE) in LDL and HDL. Active remodeling of circulating lipoproteins has been reported in the retina and retinal pigment epithelium (RPE). We previously published that CE in RPE/choroid may participate to the metabolism, and possibly uptake of dietary fatty acids in the retina, especially linoleic acid that is typically of dietary origin (Bretillon et al. Exp. Eye Res. 2008;87,521-528). In the present work, we questioned whether circulating CE fatty acid composition, LCAT and CETP activitiesmay be markers for fatty acid profile of the retina. Methods: Eyeballs were collected from nine human donors (body donation to science). The neuroretina was dissected from RPE/choroid, and a piece of the extra orbital fat was collected. Blood was sampled, and plasma was separated from red blood cells. Plasma phosphatidylcholine (PC), triglycerides and CE were prepared. The fatty acid composition of the neuroretina, phospholipids and cholesteryl esters of RPE/choroid, extra-orbital fat, plasma fractions and red blood cells was determined by gas chromatography. Estimates of LCAT and CETP activities were calculated from fatty acid data. Correlation coefficients were calculated to determine associations between adipose tissue fatty acid composition, LCAT and CETP estimates and fatty acid in the ocular structures. Results: The higher linoleic acid in plasma CE, plasma PC and adipose tissue, the greater linoleic acid in PL from RPE/choroid (r=0.96, 0.94 and 0.87, p
Published: 2009

25. Acute Postcataract Surgery Endophthalmitis Due to Streptococcus Species Differs From Endophthalmitis Due to Staphylococcus Species at Presentation

Author: Cornut, Pierre Loïc, Thuret, Gilles, Creuzot‐garcher, Catherine, Maurin, Max, Péchinot, André, Bron, Alain Marie, Romanet, Jean-Paul, Denis, Philippe, Vandenesch, François, Chiquet, Christophe, and Julien, Sabine
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, endophthalmitis, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, microbial pathogenesis: clinical studies, bacterial disease
Abstract: Purpose: : To correlate the presenting clinical ophthalmic features with the bacterial identification in 100 patients with acute post catarcat endophthalmitis enrolled in the FRench Instutionnal ENdophthalmitis Study (FRIENDS).Methods: : Demographic data, past medical history and initial eye examination were recorded in a standardized form in 100 patients with acute endophthalmitis after cataract extraction (< 6 weeks) in a prospective multicenter study. Relationship between microbiological identification (using conventional cultures and panbacterial PCR) and clinical factors at baseline was studied using univariate (ANOVA).Results: : 100 patients were hospitalized for endophthalmitis treatment with a mean delay of 8±7.5 (SD) days (range: 2-31 days) after the cataract surgery, complicated by capsular rupture in 19% of the cases. Main symptoms were loss of vision (95%) frequently limited to light perception (32%), and pain (75%). More frequent clinical signs were hypopion (72%), cyclitic membrane (77.5%) and absence of fundus visibility (90%). As compared with Staphylococcus species, the identification of Streptococcus species was more frequently identified (p
Published: 2009

26. RPE/Choroid and Neuroretina Differences in Lipid Profile in Humans, and Possible Correlations to Dietary Lipid Intake

Author: Brétillon, Lionel, Thuret, Gilles, Grégoire, Stéphane, Acar, Niyazi, Joffre, Corinne, Bron, Alain, Gain, Philippe, Creuzot Garcher, Catherine, ProdInra, Migration, Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet [Saint-Étienne] (UJM), and Association for Research in Vision and Ophthamology (ARVO). USA.
Subjects: lipids, [SDV] Life Sciences [q-bio], retina, genetic structures, nutritional factors, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, lipids (amino acids, peptides, and proteins), [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, sense organs, [SDV.IDA] Life Sciences [q-bio]/Food engineering, eye diseases
Abstract: International audience; Purpose: : To provide data on the differences in the lipid profile of the neural retina and RPE/choroid in healthy human eyes, and to establish plausible relationships with dietary fat intake, by comparing the lipid profile of the extra orbital fat. Methods: : Human eyes were collected from 28 human donors. The neural retina was dissected from the RPE/choroid. In 6 donors, the lacrimal gland and a piece of the extra orbital fat were collected. The lipid profile of the human samples was analyzed into classes. Phospholipids and cholesteryl esters were purified from RPE/choroid. The fatty acid composition of the retina, lipid fractions of RPE/choroid, and lacrimal gland samples was determined by gas chromatography and compared to the extra-orbital fat. Results: : Cholesteryl esters accounted for the major difference between the neuroretina and RPE/choroid, 1.7 ± 0.6% and 19.0 ± 3.4% of total lipids, respectively. A positive relationship was found between DHA levels in the neuroretina and cholesteryl esters from RPE/choroid (rPearson=0.78, p=0.01), while the opposite was observed in phospholipids from RPE/choroid (rPearson=-0.71, p=0.03). DHA levels in extra-orbital fat was positively correlated to DHA in lacrimal gland (rPearson=0.74, p=0.02), whereas no significant correlation was observed in the other ocular structures. Linoleic acid in extra-orbital fat and in the ocular structures were positively correlated each others, the greatest relationship was obtained with the lacrimal gland (rPearson=0.99, p
Published: 2008

27. Lipid composition of lacrimal glands in rats: comparison with human lacrimal glands and possible nutritional modulation

Author: Joffre, Corinne, Viau, Sabrina, Grégoire, Stéphane, Juaneda, Pierre, Pasquis, Bruno, Thuret, Gilles, Schnebelen, Coralie, Bron, Alain, Creuzot Garcher, Catherine, Brétillon, Lionel, FLAveur, VIsion et Comportement du consommateur (FLAVIC), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Université de Bourgogne (UB), Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Association for Research in Vision and Ophthamology (ARVO). USA., and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], lipids, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, inflammation, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, lipids (amino acids, peptides, and proteins), [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, lacrimal gland
Abstract: International audience; Purpose:: Prostaglandins (PGs) have been found in tears of patients with ocular surface disorders, and are powerful markers of inflammation. The lacrimal gland has been reported to synthesize PGs. Polyunsaturated fatty acids esterified in phospholipids are the precursors of pro- and anti-inflammatory PGs. The aim of this study was to compare the phospholipid content of the lacrimal gland in human and in different rat species. Moreover the changes in fatty acids of the lacrimal glands of Lewis rats were studied after a nutritional intervention. Methods:: Lacrimal glands were excised from Wistar, Sprague-Dawley and Lewis rats (3 month-old) and from Humans 3 to 36 hours after death. Phospholipids were extracted, quantified and separated by HPLC. Phospholipid FAs were analyzed by gas chromatography. Results:: Whereas phospholipids represented the major lipid class in Wistar and Sprague-Dawley rats (73-75%), they accounted for 50% of total lipids in Lewis rats and only 4% in Humans. Phospholipids in Humans contained 40% phosphatidylethanolamine as in Lewis and Sprague Dawley, significantly less phosphatidylcholine and more phosphatidylserine than the 3 rat species (35% versus 41-43% phosphatidylcholine and 8.0% versus 4-5% phosphatidylserine). In the 3 rat species, phospholipids contained 3-5% of n-3 fatty acids and 29-37% of n-6 fatty acids with a ratio n-6/n-3 ranging from 6 to 9. In human, their content varied from one individual to another, probably depending on the diet of the patient, but was comparable to Lewis and Sprague-Dawley rats. A diet enriched with n-3 fatty acids EPA (20:5 n-3) and DHA (C22:6n-3) given to Lewis rats for 2 months significantly increased C20:5n-3 (from 0.3 to 10.8%), C22:5n-3 and C22:6n-3 and decreased C20:4n-6 (from 17.5 to 12.0%), leading to a significant decrease in the n-6/n-3 ratio (from 5.6 to 1.3). Conclusions:: Based on the similarity between Humans and Lewis rats in terms of fatty acid profile of the lacrimal glands and on the ability of the lacrimal glands to dietary changes, dietary fatty acids may be powerful to modulate inflammatory processes in the lacrimal glands in humans, and possibly to reduce ocular surface disorders characterized by inflammation.
Published: 2007

28. Morphométrie endothéliale par analyse d'image des cornées conservées en organoculture : étude prospective de 505 cornées consécutives Endothelial morphometry of organ cultured corneas using image analysis: prospective study of 505 consecutive corneas

Author: Thuret, Gilles, Defreyn, Alexandre, Acquart, Sophie, Deb-Joardar, Nilanjana, Zhao, Min, Gavet, Yann, Theilliere, C., Pereira, S., Peoc'H, Michel, Garraud, Olivier, Maugery, Jean, Gain, Phillipe, Service d'ophtalmologie [CHU de Saint-Etienne] (SAINTOPH), CHU Saint-Etienne, Etablissement Français du Sang (EFS), EFS, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Imagerie et Morphologie (DIM-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CIS, Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-IFR143, Département de pathologie, CHU Saint-Etienne-Hôpital Bellevue, Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang, Université Jean Monnet Saint Etienne, and CHU de Saint-Etienne
Subjects: cellules hexagonales, organoculture, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, cornées, densité cellulaire endothéliale, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, coefficient de variation de surface cellulaire (CV), analyse d'image, morphométrie endothéliale, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; But Déterminer les facteurs influençant la morphométrie endothéliale des cornées en organoculture (mesure par analyse d'image) : coefficient de variation de surface cellulaire (CV) et pourcentage de cellules hexagonales. Matériels et Méthodes L'endothélium de 505 cornées consécutives conditionnées à la banque de cornée de l'EFS Loire/Auvergne a été analysé en routine par l'analyseur Sambacornea sur 3 images grand champ, après dilatation osmotique des espaces intercellulaires et comptage d'au moins 300 cellules. La qualité du set d'images était classée en mauvaise, acceptable, ou bonne par un observateur indépendant. Paramètres étudiés : âge et sexe du donneur, statut cristallinien, délai postmortem, type de milieu de conservation, durée de conservation avant analyse, qualité intrinsèque des images saisies. Résultats L'analyse d'image a été possible sur 504 des 505 cornées évaluées. L'âge du donneur était significativement corrélé à la densité cellulaire endothéliale (r =-0343) au CV (r = 0,221) et à l'hexagonalité (r =-0,314) (p < 0,001 pour les 3). La qualité des images influençait significativement ces 3 paramètres, DCE et hexagonalité baissant parallèlement à la qualité des images tandis que le CV augmentait. Pour les 258 cornées évaluées 2 fois (en moyenne à J + 4 puis à J + 14) DCE, CV et hexagonalité diminuaient en cours de conservation. Il n'existait pas d'influence du milieu sur la morphométrie. Discussion Malgré les conditions difficiles de l'organoculture et la qualité des images de microscopie optique parfois médiocre, l'analyse par Sambacornea de l'endothélium des greffons fournit des paramètres endothéliaux qui sont cliniquement fiables puisque superposables à celles connues de longue date en microscopie spéculaire. La morphométrie (CV et hexagonalité) est susceptible d'apporter des informations supplémentaires sur la fonction endothéliale, peut être en particulier pour les greffons proches du seuil conventionnel de cession de 2000 cell/mm2. Conclusion Des essais cliniques prospectifs utilisant l'analyse d'image devront évaluer l'influence de la morphométrie endothéliale sur la survie du greffon à long terme chez le receveur.
Published: 2007

29. Comparaison entre 3 microscopes spéculaires non-contact Comparison between 3 non-contact specular microscopes

Author: Gain, Phillipe, Nguyen, Frédéric, Deb-Joardar, Nilanjana, Zhao, Min, Gavet, Yann, Dumollard, Jean-Marc, Peoc'H, Michel, Maugery, Jean, Thuret, Gilles, Service d'ophtalmologie [CHU de Saint-Etienne] (SAINTOPH), CHU Saint-Etienne, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Imagerie et Morphologie (DIM-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CIS, Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-IFR143, Department of Pathology, Saint Etienne University Hospital, Service de neuropathologie, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet Saint Etienne, and CHU de Saint-Etienne
Subjects: [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, détection des centres cellulaires, microscopes spéculaires (MS) non-contact, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, méthode de Bland Altman, densité cellulaire endothéliale (DCE), [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; Introduction Les microscopes spéculaires (MS) non-contact Rhinetec®, SeaEagle® pour la station complète d'acquisition et de traitement de l'image et SeaEagle SL® pour la version simplifiée adaptable sur lampe à fente, mesurent la densité cellulaire endothéliale (DCE) par détection des centres cellulaires soit automatiquement soit après retouche manuelle. But : déterminer leur concordance avec le MS non-contact Topcon SP2000®, le plus utilisé, mesurant la DCE par détection des contours après retouche obligatoire. Matériels et Méthodes Analyse de la DCE centrale de 270 (SeaEagle®) et de 108 cornées (SeaEagle SL®). Utilisation de la méthode de Bland Altman pour déterminer la concordance entre le comptage retouché de Topcon® (gold standard) et les comptages automatiques puis retouchés de SeaEagle® et SeaEagle SL®. Résultats Pour SeaEagle®. Nombre de cellules prises en compte peu différent (moyenne, écart-type) : 137 (44) vs 129 (46) (Topcon®) (p < 0.001). Faible concordance entre comptage automatique SeaEagle® et comptage retouché Topcon®, avec une moyenne des différences de +226 cell/mm2 de surestimation globale de SeaEagle ® (limites de -663 à +1 116 cell/mm2). Par contre, bonne concordance entre les deux comptages retouchés avec une moyenne des différences de seulement -2 cell/mm2 (limites de -412 à +408 cell/mm2) pour SeaEagle®. Pour ®SeaEagle SL (mode retouché uniquement). Nombre de cellules prises en compte peu différent : 115 (30) vs 132 (43) (Topcon®) (p < 0.001). Concordance moins bonne entre les deux comptages retouchés avec une moyenne des différences de +103 cell/mm2 (limites de -286 à +493 cell/mm2) pour SeaEagle SL®. Discussion Les comptages automatiques de SeaEagle® et SeaEagle SL® ne sont pas concordants avec le comptage retouché de Topcon®. Les comptages retouchés sont concordants, même s'il peut ponctuellement exister une variation l'ordre de 400 à 500 cell/mm2. Conclusion Le comptage automatique doit être banni des bonnes pratiques de comptage endothélial. Le comptage retouché avec SeaEagle® a une concordance excellente avec Topcon® retouché, moindre dans sa version adaptable sur lampe à fente SeaEagle SL®. Cette dernière nous paraît néanmoins acceptable pour une utilisation de routine, hors recherche clinique.
Published: 2007

30. Concordance des pachymétries contact ultrasonique, non contact spéculaire et non contact par réflectométrie en basse cohérence optique Agreement of pachymetry measurements using ultrasound, two non contact specular microscopes and optical low-coherence reflectometry

Author: Nguyen, Frédéric, Thuret, Gilles, Deb-Joardar, Nilanjana, Zhao, Min, Gavet, Yann, Dumollard, Jean-Marc, Peoc'H, Michel, Maugery, J., Gain, Phillipe, Service d'ophtalmologie [CHU de Saint-Etienne] (SAINTOPH), CHU Saint-Etienne, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Imagerie et Morphologie (DIM-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CIS, Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-IFR143, Department of Pathology, Saint Etienne University Hospital, Service de neuropathologie, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang, Université Jean Monnet Saint Etienne, and CHU de Saint-Etienne
Subjects: épaisseur cornéenne centrale, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, microscope spéculaire monté sur lampe à fente, réflectomètre en basse cohérence optique (RBCO) non-contact, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, méthode de Bland Altman, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, microscope spéculaire non-contact, pachymètre ultrasonore contact
Abstract: International audience; But Le but de cette étude était de comparer les mesures d'épaisseur cornéenne centrale obtenues par 4 méthodes : réflectomètre en basse cohérence optique (RBCO) non-contact, monté sur lampe à fente, 2 microscopes spéculaires non-contact, une version simplifiée d'un microscope spéculaire monté sur lampe à fente et un pachymètre ultrasonore contact comme gold standard Matériels et Méthodes L'épaisseur cornéenne a été mesurée à l'apex cornéen de 108 yeux de 54 adultes (72 % de volontaires sains, 22 % de greffés de la cornée, 6 % d'opérés de cataracte) avec les 3 dispositifs non-contact dans un ordre aléatoire (moyenne de 10 mesures pour la RBCO, mesure unique pour les microscopes spéculaires). La mesure par ultrason était réalisée en dernier (moyenne de 10 mesures). Le nombre des mesures reflétait la pratique courante. La concordance entre chacune des techniques non-contact et le gold standard était mesurée par la méthode de Bland Altman en déterminant les différences moyennes entre les techniques et leurs deux bornes de concordances Résultats L'épaisseur centrale (moyenne ± écart-type) était de 541 ± 37 μm pour l'ultrason, 539 ± 38 pour la RBCO (P = 0.45), 528 ± 39 pour Topcon (P < 0,01) et 564 ± 67 pour Rhinetec (P < 0.01). La différence moyenne avec la pachymétrie ultrasonore et les bornes de concordances étaient de – 2 μm [− 24 ; + 19] pour l'OBCR, − 12 μm [− 48 ; + 24] pour Topcon, et + 23 μm [− 92 ; + 139] pour Rhinetec. Discussion Les concordances avec la pachymétrie ultrasonore de la RBCO et de Topcon apparaissent excellentes, tandis qu'elle n'est pas suffisante pour Rhinetec. Topcon sous estime très modérément l'épaisseur. La RBCO semble la plus fiable. Conclusion La RBCO peut être utilisée de façon interchangeable avec la pachymétrie ultrasonore. En plus de son utilisation en routine clinique, elle pourrait s'avérer utile pour mesurer l'épaisseur des greffons cornéens sans risque de contamination au cours de l'organoculture, dans le but de dépister les chirurgies réfractives cornéennes.
Published: 2007

31. Stratégies de comptage par analyse d'image de l'endothélium des cornées conservées en organoculture : détection de contour versus pointage du centre . Counting strategies for endothelial assessment of organ cultured corneas using image analysis: comparison of border versus center method

Author: Thuret, Gilles, Deb-Joardar, Nilanjana, Zhao, Min, Robert, Pierre-Yves, Gavet, Yann, Acquart, Sophie, Chavarin, Patricia, Dumollard, Jean-Marc, Peoc'H, Michel, Maugery, Jean, Gain, Phillipe, Service d'ophtalmologie [CHU de Saint-Etienne] (SAINTOPH), CHU Saint-Etienne, Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), Biologie moléculaire et cellulaire des microorganismes (EA3175), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Limoges (UNILIM), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Imagerie et Morphologie (DIM-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CIS, Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-IFR143, Etablissement Français du Sang (EFS), EFS, Auvergne-Loire Regional Blood Bank (EFS Auvergne-Loire), Saint-Etienne, Ministère de la santé, Department of Pathology, Saint Etienne University Hospital, Département de pathologie, CHU Saint-Etienne-Hôpital Bellevue, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang, Université Jean Monnet Saint Etienne, CHU de Saint-Etienne, CHU Limoges, and Université de Limoges
Subjects: border methods, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, endothelial cell density (ECD), coefficient of variation (CV), cell count strategies, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, endothelial assessment, center methods, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, corneal storage
Abstract: International audience; Aim To compare two different cell count strategies, border and center methods for endothelial assessment during corneal storage. Material and Methods Seven observers determined the endothelial cell density (ECD), coefficient of variation (CV) of cell area, and percentage hexagonality of 30 organ cultured corneas by the border (contour detection and manual retouch) and center method (indicating cell centers) using Sambacornea analyser. Interobserver variability for ECD and agreement between the two methods was determined. The accuracy of the center method was verified by counting on ten standard photolithographic mosaics (called keratotest) by border (reference method) and center methods, first as fast and next as precise as possible (fast and slow modes respectively) and the time noted. Data between border and center methods was compared using nonparametric paired tests. Results For stored corneas, interobserver variability was ±9.6% (95%CI [6.5-12.7]) for border method and ±9.3% (95%CI[6.3-12.3]) for center method. ECD [mean±SD (range), median] was [2948±565 (1644-3878), 3081] and [2961±568 (1736-3914), 3037] respectively and showed excellent correlation (Pearson coefficient r=0.998, p
Published: 2007

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31 results on '"Thuret, Gilles"'

1. Histological and in situ microscopic observation of femtosecond laser induced incisions in the crystalline lens

2. Birth and death simulation of human corneal endothelium cells

3. 4812 - A0028 Gangliosides in the retina: a comparative inventory with brain and other ocular structures

4. Gangliosides in the retina: a comparative inventory with brain and other ocular structures

5. Delivery of macromolecules into the endothelium of whole ex vivo human cornea by femtosecond laser-activated carbon nanoparticles

6. Associations between erythrocyte and ciliary epithelium phosphatidylcholine concentrations in healthy humans

7. Longitudinal study of retinal status using optical coherence tomography after acute post-cataract endophthalmitis

8. Delivery of molecules into human corneal endothelial cells by carbon nanoparticles activated by femtosecond laser

9. Dietary fatty acids and the prevention of Age-related Macular Degeneration: retinal incorporation and beyond

10. Femtosecond laser activated carbon nanoparticles for corneal gene therapy

11. Effect of an adjunctive first-line intravitreal betamethasone injection in postoperative endophthalmitis

12. Circulating markers of retinal and optic nerve lipids

13. Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages.: DLL4's opposing effects in choroidal neovascularization

14. Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages. : DLL4's opposing effects in choroidal neovascularization

15. Relationship between baseline clinical data and microbiologic spectrum in 100 patients with acute postcataract endophthalmitis

16. Study of stromal femtosecond laser ablation for deep corneal cut optimization

17. Decreasing the dietary intake of linoleic acid promotes the efficacy of dietary long chain omega-3 fatty acids to incorporate the rat retina and modifies gene expression

18. Friends group: Post trauma endophthalmitis

19. Mesure d'épaisseur de lentilles de contact et détection de traces de chirurgie réfractive dans des cornées par interférométrie à faible longueur de cohérence

20. Quantitative analysis of fatty acids on choline- and ethanolamine-phospholipids in human ocular and non-ocular tissues by liquid chromatography/tandem mass spectrometry (LC-ESI-MS/MS)

21. Factors of good visual outcome in acute post-cataract endophthalmitis (friends group)

22. Analysis of diluted vitreous samples from vitrectomy is useful in eyes with severe acute postoperative endophthalmitis

23. A new HPLC-ESI-MS/MS method to characterize and quantify phosphatidyl-choline with VLC-PUFA: Application to human retina

24. Would circulating cholesteryl esters be considered as markers for fatty acid composition of the human retina?

25. Acute Postcataract Surgery Endophthalmitis Due to Streptococcus Species Differs From Endophthalmitis Due to Staphylococcus Species at Presentation

26. RPE/Choroid and Neuroretina Differences in Lipid Profile in Humans, and Possible Correlations to Dietary Lipid Intake

27. Lipid composition of lacrimal glands in rats: comparison with human lacrimal glands and possible nutritional modulation

28. Morphométrie endothéliale par analyse d'image des cornées conservées en organoculture : étude prospective de 505 cornées consécutives Endothelial morphometry of organ cultured corneas using image analysis: prospective study of 505 consecutive corneas

29. Comparaison entre 3 microscopes spéculaires non-contact Comparison between 3 non-contact specular microscopes

30. Concordance des pachymétries contact ultrasonique, non contact spéculaire et non contact par réflectométrie en basse cohérence optique Agreement of pachymetry measurements using ultrasound, two non contact specular microscopes and optical low-coherence reflectometry

31. Stratégies de comptage par analyse d'image de l'endothélium des cornées conservées en organoculture : détection de contour versus pointage du centre . Counting strategies for endothelial assessment of organ cultured corneas using image analysis: comparison of border versus center method

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