1. Regulated tissue fluidity steers zebrafish body elongation
- Author
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Thierry Emonet, Andrew K. Lawton, Michael W. Sneddon, Nicolas Dray, Scott A. Holley, Michael J. Stulberg, Amitabha Nandi, William Pontius, Yale University [New Haven], Research support was provided by a National Institutes of Health (NIH) predoctoral Developmental Biology training grant [T32 HD07180-29 to A.K.L.], a NIH predoctoral Genetics training grant [T32 GM007499 to M.J.S.], the National Institute of Child Health and Human Development (NICHD) [RO1 HD045738 to S.A.H.], a Research Scholar Grant from the American Cancer Society [to S.A.H.], the Raymond and Beverly Sackler Institute for Biological, Physical and Engineering Sciences [T.E. and S.A.H.], the James McDonnell Foundation [T.E.], and by the facilities and staff of the Yale University Faculty of Arts and Sciences High Performance Computing Center. Deposited in PMC for release after 12 months., We thank Joe Wolenski for microscopy support, and and Jamie Schwendinger-Schreck, Bryan Leland and Patrick McMillen for comments on the manuscript.
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Embryo, Nonmammalian ,Time Factors ,Tailbud ,[SDV]Life Sciences [q-bio] ,Cell ,Cell Count ,MESH: Cadherins/genetics ,MESH: Embryo, Nonmammalian/cytology ,MESH: Fibroblast Growth Factors/metabolism ,Animals, Genetically Modified ,0302 clinical medicine ,Cell Movement ,MESH: Zebrafish/metabolism ,MESH: Embryonic Development ,MESH: Animals ,Zebrafish ,Wnt Signaling Pathway ,Research Articles ,0303 health sciences ,MESH: Cadherins/metabolism ,MESH: Fibroblast Growth Factors/genetics ,Wnt signaling pathway ,MESH: Wnt Signaling Pathway ,Cell Polarity ,Gene Expression Regulation, Developmental ,Cadherins ,Cell biology ,Biomechanical Phenomena ,medicine.anatomical_structure ,MESH: Zebrafish/genetics ,MESH: Embryo, Nonmammalian/metabolism ,MESH: Cell Polarity ,MESH: Tail/embryology ,Tail ,Leading edge ,Axial skeleton ,MESH: Cell Movement ,MESH: Biomechanical Phenomena ,MESH: Tail/metabolism ,Embryonic Development ,Body elongation ,Biology ,Models, Biological ,MESH: Cell Adhesion ,MESH: Animals, Genetically Modified ,03 medical and health sciences ,MESH: Computer Simulation ,MESH: Zebrafish Proteins/genetics ,medicine ,Cell Adhesion ,Animals ,Computer Simulation ,Cell migration ,MESH: Zebrafish/embryology ,Progenitor cell ,MESH: Body Patterning ,MESH: Gene Expression Regulation, Developmental ,Molecular Biology ,030304 developmental biology ,Body Patterning ,MESH: Zebrafish Proteins/metabolism ,Cadherin ,MESH: Cell Count ,MESH: Time Factors ,MESH: Models, Biological ,Zebrafish Proteins ,Spinal cord ,biology.organism_classification ,Fibroblast Growth Factors ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
International audience; The tailbud is the posterior leading edge of the growing vertebrate embryo and consists of motile progenitors of the axial skeleton, musculature and spinal cord. We measure the 3D cell flow field of the zebrafish tailbud and identify changes in tissue fluidity revealed by reductions in the coherence of cell motion without alteration of cell velocities. We find a directed posterior flow wherein the polarization between individual cell motion is high, reflecting ordered collective migration. At the posterior tip of the tailbud, this flow makes sharp bilateral turns facilitated by extensive cell mixing due to increased directional variability of individual cell motions. Inhibition of Wnt or Fgf signaling or cadherin 2 function reduces the coherence of the flow but has different consequences for trunk and tail extension. Modeling and additional data analyses suggest that the balance between the coherence and rate of cell flow determines whether body elongation is linear or whether congestion forms within the flow and the body axis becomes contorted.
- Published
- 2013
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