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5 results on '"Xiaopeng, Fu"'

1. Tissue-Specific Expression of the Terpene Synthase Family Genes in Rosa chinensis and Effect of Abiotic Stress Conditions

Author

Yuhang Yan, Mouliang Li, Xiaoni Zhang, Weilong Kong, Mohammed Bendahmane, Manzhu Bao, Xiaopeng Fu, Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University [Wuhan] (HZAU), Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-16-CE20-0024,ROSASCENT,Biosynthèse des terpènes du parfum chez la rose(2016)

Subjects
Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Genetics (clinical), rose, terpene synthase, genome-wide analysis, expression pattern, abiotic stress
Abstract

International audience; Rose (Rosa chinensis) is one of the most famous ornamental plants worldwide, with a variety of colors and fragrances. Terpene synthases (TPSs) play critical roles in the biosynthesis of terpenes. In this work, we report a comprehensive study on the genome-wide identification and characterization of the TPS family in R. chinensis. We identified 49 TPS genes in the R. chinensis genome, and they were grouped into five subfamilies (TPS-a, TPS-b, TPS-c, TPS-g and TPS-e/f). Phylogenetics, gene structure and conserved motif analyses indicated that the RcTPS genes possessed relatively conserved gene structures and the RcTPS proteins contained relatively conserved motifs. Multiple putative cis-acting elements involved in the stress response were identified in the promoter region of RcTPS genes, suggesting that some could be regulated by stress. The expression profile of RcTPS genes showed that they were predominantly expressed in the petals of open flowers, pistils, leaves and roots. Under osmotic and heat stresses, the expression of most RcTPS genes was upregulated. These data provide a useful foundation for deciphering the functional roles of RcTPS genes during plant growth as well as addressing the link between terpene biosynthesis and abiotic stress responses in roses.

Published
2022
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2. Regeneration and Agrobacterium-mediated genetic transformation in Dianthus chinensis

Author

Zhen Zhang, Mohammed Bendahmane, Quanshu Wu, Xiuli Yan, Manzhu Bao, Shengnan Lin, Xiaopeng Fu, Qijian Wang, Xiaoni Zhang, Zehao Wang, Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University [Wuhan] (HZAU), College of Plant Science & Technology, Huazhong Agriculture University, Wuhan 430070, China, Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Key Laboratory of East China Urban Agriculture, Ministry of Agriculture of the People's Republic of China (MOA), Chinese Academy of Agricultural Sciences (CAAS), and National Natural Science Foundation of China (NSFC)31872135Fundamental Research Funds for the Central Universities2662018JC036

Subjects
0106 biological sciences, 0301 basic medicine, food.ingredient, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Agrobacterium, Horticulture, Biology, 01 natural sciences, Genetic transformation, 03 medical and health sciences, food, Dianthus, Botany, medicine, Regeneration, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, A. Tumefaciens, Regeneration (biology), fungi, food and beverages, Kanamycin, Agrobacterium tumefaciens, biology.organism_classification, Transformation (genetics), 030104 developmental biology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Cotyledon, Dianthus chinensis, 010606 plant biology & botany, medicine.drug
Abstract

International audience; Dianthus chinensis is widely used as a landscaping plant in rock gardens and has high commercial value. However, few studies of this plant's regeneration and genetic transformation have been reported. In this study, cotyledons of D. chinensis were used as explants for regeneration and infection with Agrobacterium. The results indicated that the efficiency of regeneration was markedly improved by using 1-naphthylcetic acid (NAA). The cotyledons were cocultured with Agrobacterium tumefaciens EHA105 containing pK7GWIWG2-GFP plasmids for 15-20 min. Transgenic regenerated plants were obtained by kanamycin (80 mg.L-1) screening. qRT-PCR results confirmed the expression of GFP and the DcIPT1 (which is related to cell division) gene in the transgenic DcIPT1 plants of D. chinensis. The results show that the proliferation ability of transgenic DcIPT1 plants is enhanced. Overall, we report the successful establishment of a reliable and efficient method for cotyledon transformation and plant regeneration in D. chinensis based on the combination of infection with Agrobacterium and plant regeneration. This work will contribute to functional gene research and genetic improvement of Dianthus species plants.

Published
2021
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3. Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis

Author

Xiaopeng Fu, Xiaoni Zhang, Shengnan Lin, Mohammed Bendahmane, Manzhu Bao, Shaozong Yang, Xiuli Yan, Qijian Wang, Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University [Wuhan] (HZAU), Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and National Natural Science Foundation of China (NSFC)31872135Fundamental Research Funds for the Central Universities2662018JC036

Subjects
0106 biological sciences, 0301 basic medicine, QTL mapping, Candidate gene, Genetic Linkage, Physiology, Single-nucleotide polymorphism, Flowers, Plant Science, Biology, Quantitative trait locus, Polymorphism, Single Nucleotide, 01 natural sciences, 03 medical and health sciences, ddRAD, Dianthus, Genotype, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, double flower trait, miR172, [SDV.BDD]Life Sciences [q-bio]/Development Biology, 2. Zero hunger, Genetics, BSR-seq, AcademicSubjects/SCI01210, fungi, Chromosome Mapping, food and beverages, biology.organism_classification, Research Papers, Phenotype, 030104 developmental biology, [SDE]Environmental Sciences, Dianthus chinensis, Petal, Growth and Development, Flower formation, APETALA2 (DcAP2L), 010606 plant biology & botany
Abstract

An SNP mutation in the miR172 target site of the DcAP2L gene is associated with a double flower phenotype in Dianthus chinensis., The double flower is a highly important breeding trait that affects the ornamental value in many flowering plants. To get a better understanding of the genetic mechanism of double flower formation in Dianthus chinensis, we have constructed a high-density genetic map using 140 F2 progenies derived from a cross between a single flower genotype and a double flower genotype. The linkage map was constructed using double-digest restriction site-associated DNA sequencing (ddRAD-seq) with 2353 single nucleotide polymorphisms (SNPs). Quantitative trait locus (QTL) mapping analysis was conducted for 12 horticultural traits, and major QTLs were identified for nine of the 12 traits. Among them, two major QTLs accounted for 20.7% and 78.1% of the total petal number variation, respectively. Bulked segregant RNA-seq (BSR-seq) was performed to search accurately for candidate genes associated with the double flower trait. Integrative analysis of QTL mapping and BSR-seq analysis using the reference genome of Dianthus caryophyllus suggested that an SNP mutation in the miR172 cleavage site of the A-class flower organ identity gene APETALA2 (DcAP2L) is responsible for double flower formation in Dianthus through regulating the expression of DcAG genes.

Published
2020
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4. The Rosa genome provides new insights into the domestication of modern roses

Author

Sylvie Baudino, Caroline Pont, Arnaud Lemainque, Michiel Vandenbussche, Jean-Claude Caissard, Teva Vernoux, Patrick Wincker, Florence Piola, Chang Liu, Hélène Badouin, Adnane Boualem, Mohammed-Amin Madoui, Moussa Benhamed, Jean-Marc Aury, Jérôme Salse, Abdelhafid Bendahmane, Manuel Le Bris, Benjamin Govetto, Hadi Quesneville, Olivier Raymond, Jérôme Gouzy, Mohammed Bendahmane, Sandrine Moja, Annick Dubois, Judit Szécsi, Priscilla Villand, Claudia Bardoux, Shu-Hua Yang, Céline Lopez-Roques, Ludovic Cottret, Pascal Heitzler, Antoine Larrieu, Véronique Boltz, Nathalie Choisne, Yoan Labrousse, Léa François, David Latrasse, Marion Verdenaud, Lauriane Perrier, Philippe Vergne, Sébastien Carrère, Magali Perez, Karine Labadie, Arnaud Couloux, Xiaopeng Fu, Jérémy Just, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Sexe et évolution, Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Centre National de Génotypage (CNG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), Université Jean Monnet [Saint-Étienne] (UJM), Unité de Recherche Génomique Info (URGI), Institut National de la Recherche Agronomique (INRA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Zentrum für Molekularbiologie der Pflanzen (ZMBP), Eberhard Karls Universität Tübingen, ANR-13-BSV7-0014,DODO,Identification et caractérisation des mutations DOMINANT DOUBLE (DODO) and DOUBLE FLOWER (DF) chez le pétunia et la rose(2013), ANR-12-BSV6-0005,AuxiFlo,Couplage entre le réseau transcriptionnel de l'auxine et l'identité florale(2012), ANR-11-IDEX-02/10-LABX-0040,SPS,Saclay Plant Sciences(2011), European Project: 341076,EC:FP7:ERC,ERC-2013-ADG,SEXYPARTH(2014), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Équipe 2 - Écologie Végétale et Zones Humides (EVZH), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, GET-PACBIO program (programme operationnel FEDER-FSE MIDI-PYRENEES ET GARONNE), French National Institute of Agronomic Research (INRA), program Fonds Recherche of Ecole Normale Superieure-Lyon-France, Genoscope, French National Research Agency program DODO : ANR-16CE20-0024-03, French National Research Agency program AUXIFLO : ANR-12-BSV6-0005, Labex Saclay Plant Sciences-SPS : ANR-10- LABX-0040-SPS, ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (BVPAM), Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), and Huazhong Agricultural University [Wuhan] (HZAU)

Subjects
0301 basic medicine, Candidate gene, Genotype, Flowers, Biology, Genes, Plant, Rosa, Genome, Article, Domestication, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, 03 medical and health sciences, Gene Expression Regulation, Plant, Genetic variation, Genetics, rosaceae, Rosa chinensis, Gene, odeur, couleur, Hybrid, Plant Proteins, Vegetal Biology, Whole Genome Sequencing, génome, Genetic Variation, Genomics, Sequence Analysis, DNA, biology.organism_classification, 030104 developmental biology, Evolutionary biology, [SDE]Environmental Sciences, gène candidat, Ploidy, séquençage adn, Biologie végétale, Genome, Plant
Abstract

Epub ahead of print; International audience; Roses have high cultural and economic importance as ornamental plants and in the perfume industry. We report the rose whole-genome sequencing and assembly and resequencing of major genotypes that contributed to rose domestication. We generated a homozygous genotype from a heterozygous diploid modern rose progenitor, Rosa chinensis ‘Old Blush’. Using single-molecule real-time sequencing and a meta-assembly approach, we obtained one of the most comprehensive plant genomes to date. Diversity analyses highlighted the mosaic origin of ‘La France’, one of the first hybrids combining the growth vigor of European species and the recurrent blooming of Chinese species. Genomic segments of Chinese ancestry identified new candidate genes for recurrent blooming. Reconstructing regulatory and secondary metabolism pathways allowed us to propose a model of interconnected regulation of scent and flower color. This genome provides a foundation for understanding the mechanisms governing rose traits and should accelerate improvement in roses, Rosaceae and ornamentals.

Published
2018
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5. Genome-wide identification and characterization of aquaporin gene family in beta vulgaris

Author

Yulu Wang, Shaozong Yang, Mohammed Bendahmane, Weilong Kong, Xiaopeng Fu, Huazhong Agricultural University, Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), National Natural Science Foundation of China 31000918, Fundamental Research Funds for the Central Universities 2662015PY052 2662016PY041, Huazhong Agricultural University [Wuhan] (HZAU), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Subfamily, [SDV]Life Sciences [q-bio], Protein domain, Aquaporin, lcsh:Medicine, Aquaporins, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Expression profile, Botany, Gene family, profil d'expression, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene, 2. Zero hunger, Genetics, stress abiotique, Water transport, biology, Abiotic stress, General Neuroscience, lcsh:R, aquaporine, General Medicine, biology.organism_classification, Gene structure, 030104 developmental biology, beta vulgaris, Sugar beet, General Agricultural and Biological Sciences, 010606 plant biology & botany, gène de structure
Abstract

Aquaporins (AQPs) are essential channel proteins that execute multi-functions throughout plant growth and development, including water transport, uncharged solutes uptake, stress response, and so on. Here, we report the first genome-wide identification and characterization AQP (BvAQP) genes in sugar beet (Beta vulgaris), an important crop widely cultivated for feed, for sugar production and for bioethanol production. Twenty-eight sugar beet AQPs (BvAQPs) were identified and assigned into five subfamilies based on phylogenetic analyses: seven of plasma membrane (PIPs), eight of tonoplast (TIPs), nine of NOD26-like (NIPs), three of small basic (SIPs), and one of x-intrinsic proteins (XIPs). BvAQP genes unevenly mapped on all chromosomes, except on chromosome 4. Gene structure and motifs analyses revealed that BvAQP have conserved exon-intron organization and that they exhibit conserved motifs within each subfamily. Prediction of BvAQPs functions, based on key protein domains conservation, showed a remarkable difference in substrate specificity among the five subfamilies. Analyses of BvAQPs expression, by mean of RNA-seq, in different plant organs and in response to various abiotic stresses revealed that they were ubiquitously expressed and that their expression was induced by heat and salt stresses. These results provide a reference base to address further the function of sugar beet aquaporins and to explore future applications for plants growth and development improvements as well as in response to environmental stresses.

Published
2017
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