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28 results on '"Zhang, Man"'

3. Isolation and Characterization of Carbapenem-Resistant Escherichia coli Carrying blaNDM and mcr-1 from Recurrent Urinary Tract Infection Patient.

Author

Chen, Liang, Jian, Jiyong, Xie, Zeqiang, Zhao, Ping, and Zhang, Man

Subjects
URINARY tract infections, ESCHERICHIA coli, MICROBIAL sensitivity tests, MEMBRANE proteins
Abstract

Objective. The emergence of carbapenem-resistant E. coli (CRECO), leading to few antibacterial drugs available for CRECO infection. In this study, we report three carbapenem-resistant Escherichia coli (E. coli) isolates coproducing blaNDM and mcr-1 from patients with recurrent urinary tract infection (RUTI). Carbapenem-resistant E. coli strains, E55, E84, and E85, were isolated from the urine sample of RUTI patients. Methods. Antimicrobial susceptibility testing (AST) was conducted with VITEK-2 compact system and Kirby–Bauer (K-B) disk diffusion method. The ESBL test was detected by the disk diffusion method. The EDTA-modified carbapenem inactivation method (eCIM) and modified carbapenem inactivation method (mCIM) were performed for screening the carbapenemase. Multilocus sequence typing (MLST) was performed for molecular typing of the strains. The resistance genes were detected by PCR. Results. The three isolates were all susceptible to tigecycline and nitrofurantoin. The blaNDM-1, blaCMY-6, blaTEM-1 and blaCTX-M-1, mcr-1, and porin loss expression of outer membrane protein F (OmpF) were detected in E55, which was assigned to ST2. The E84 and E85 were identified as ST471 carrying blaNDM-5, blaCTX-M55, and blaTEM-1 and the quinsolone-resistant genes aac(6′)-Ib-cr and mcr-1. Conclusion. To our knowledge, our study is the first to report carbapenem-resistant E. coli strains carrying blaNDM and mcr-1 from urine of the recurrent urinary tract infection patients. These E. coli strains carrying blaNDM and mcr-1 should be closely monitored. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Effects of amino acids on olfactory‐related receptors regulating appetite in silver pomfret

Author

Qijun Le, Zhang Man, Youyi Zhang, Xiaojun Yan, Gu Weiwei, Yibo Sun, Yajun Wang, Shanliang Xu, Yang Yang, Hu Jiabao, Kimran Jean Jacques, Kuang Siwen, Jiachu Sun, and Yaya Li

Subjects
chemistry.chemical_classification, Pomfret, Olfactory receptor, biology, media_common.quotation_subject, Appetite, Aquatic Science, biology.organism_classification, Amino acid, medicine.anatomical_structure, Biochemistry, chemistry, medicine, Pampus argenteus, Receptor, media_common
Published
2021

9. Detection of BRAF V600E in Fine-Needle Aspiration Samples of Thyroid Nodules by Droplet Digital PCR

Author

Lu, Sang-Yu, primary, Chen, Ying-Chao, additional, Feng, Jia-Lin, additional, Zhou, Qin-Yi, additional, Chen, Jing, additional, Zhu, Chen-Fang, additional, Guo, Miao-Miao, additional, Zhang, Man-Man, additional, Zhang, Qian-Yue, additional, Lu, Meng, additional, Yang, Liu, additional, Wu, Jing, additional, Zhao, Shuang-Xia, additional, Song, Huai-Dong, additional, and Ye, Xiao-Ping, additional

Published
2022
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23. Research on android infiltration technology based on the silent installation of an accessibility service.

Author

Sun, Haitao, Jin, Chengjie, Helu, Xiaohan, Lu, Hui, Zhang, Man, and Tian, Zhihong

Subjects
TCP/IP, ANTIVIRUS software, CELL phones, MARKETING software, TECHNOLOGY
Abstract

This work focuses mainly on the penetration test function and stability of Android smartphones under Kali 2019 and creates a stable penetration function of Android smartphones to form a strong reverse transmission control protocol connection. Using the silent installation technology supported by the Accessibility Service (integrated on a nontoxic functional platform), the Android Package that injects the penetration function successfully bypasses the common mobile phone antivirus software on the market to improve the penetration efficiency. [ABSTRACT FROM AUTHOR]

Published
2020
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26. Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes.

Author

Zhang, Man-Feng, Cai, Xiao-Lin, Jing, Kai-Peng, Pi, Xiao-Xue, Liao, Pei-Yu, Li, Shi-Jie, Wen-Li, Cai, Chuan-Chuan, Quan, Juan-Hua, Fan, Yi-Ming, and Ge-Shi

Subjects
*SEBACEOUS glands, *CELL differentiation, *APOPTOSIS, *BIOMARKERS, *BIOLOGICAL models, *CELL culture, *ELECTRON microscopy, *FLOW cytometry, *IMMUNOHISTOCHEMISTRY, *MOLECULAR biology, *PROTEINS, *STAINS & staining (Microscopy), *WESTERN immunoblotting, *PROTEOMICS, *IN vitro studies, *PHYSIOLOGY
Abstract

Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry. [ABSTRACT FROM AUTHOR]

Published
2018
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