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4 results on '"Rui Sang"'

1. Inhibitory Effects of Inonotus obliquus Polysaccharide on Inflammatory Response in Toxoplasma gondii-Infected RAW264.7 Macrophages

Author

Kexin Yan, Hongyuan Zhou, Meng Wang, Haitao Li, Rui Sang, Bingjie Ge, Xin Zhao, Chunting Li, Wei Wang, and Xuemei Zhang

Subjects
Other systems of medicine, Article Subject, genetic structures, Complementary and alternative medicine, RZ201-999, Research Article
Abstract

Our previous reports have shown that Inonotus obliquus polysaccharide (IOP) has protective effects against Toxoplasma gondii (T. gondii) infection in vivo. The aim of the present research is to explore the in vitro anti-inflammatory effects of IOP and its mechanism in RAW264.7 macrophages infected by T. gondii. In this study, it is indicated that IOP decreased the excessive secretion of inflammatory cytokines tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), IL-4, and IL-6 in T. gondii-infected RAW264.7 macrophages. IOP effectively suppressed the mRNA expression of these cytokines and chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1α (MIP-1α). Moreover, IOP inhibited the phosphorylation of inhibitor kappa B kinase α/β (IKKα/β), inhibitor κBα (IκBα), p65 in nuclear factor-kappa B (NF-κB) signaling pathway and p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase 1/2 (ERK1/2) in mitogen-activated protein kinases (MAPKs) signaling pathway. Meantime, IOP prevented NF-κB p65 and c-Jun translocation from the cytoplasm to the nucleus. Further, IOP downregulated the protein expression of toll-like receptor 2 (TLR2) and TLR4 in T. gondii-infected RAW264.7 macrophages. The above results suggest that IOP can inhibit the inflammatory response infected with T. gondii via regulating TLR2/TLR4-NF-κB/MAPKs pathways and exerting its anti-T. gondii role in vitro.

Published
2021

2. Actinobacillus succinogenes ATCC 55618 fermentation medium optimization for the production of succinic acid by response surface methodology

Author

Zhu, Li-Wen, Wang, Cheng-Cheng, Liu, Rui-Sang, Li, Hong-Mei, Wan, Duan-Ji, and Tang, Ya-Jie

Subjects
Minitab Inc., Computer software industry -- Methods -- Analysis, Dextrose -- Methods -- Analysis, Glucose -- Methods -- Analysis, Glucose metabolism -- Methods -- Analysis, Biotechnology industry, High technology industry
Abstract

As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimize Actinobacillus succinogenes ATCC 55618 fermentation medium. First, glucose, yeast extract, and MgC[O.sub.3] were identified to be key medium components by PBD. Second, preliminary optimization was run by SA method to access the optimal region of the key medium components. Finally, the responses, that is, the production of succinic acid, were optimized simultaneously by using BBD, and the optimal concentration was located to be 84.6 g [L.sup.-1] of glucose, 14.5 g [L.sup.-1] of yeast extract, and 64.7 g [L.sup.-1] of MgC[O.sub.3]. Verification experiment indicated that the maximal succinic acid production of 52.7 ± 0.8g [L.sup.-1] was obtained under the identified optimal conditions. The result agreed with the predicted value well. Compared with that of the basic medium, the production of succinic acid and yield of succinic acid against glucose were enhanced by 67.3% and 111.1%, respectively. The results obtained in this study may be useful for the industrial commercial production of succinic acid., 1. Introduction Succinic acid is a member of the four-carbon dicarboxylic acid family in tricarboxylic acid cycle (TCA) and has a wide range of applications in the fields of surfactants, [...]

Published
2012
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4. Actinobacillus succinogenes ATCC 55618 Fermentation Medium Optimization for the Production of Succinic Acid by Response Surface Methodology.

Author

Li-Wen Zhu, Cheng-Cheng Wang, Rui-Sang Liu, Hong-Mei Li, Duan-Ji Wan, and Ya-Jie Tang

Abstract

As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimize Actinobacillus succinogenes ATCC 55618 fermentation medium. First, glucose, yeast extract, and MgCO3 were identified to be key medium components by PBD. Second, preliminary optimization was run by SA method to access the optimal region of the key medium components. Finally, the responses, that is, the production of succinic acid, were optimized simultaneously by using BBD, and the optimal concentration was located to be 84.6 g L-1 of glucose, 14.5 g L-1 of yeast extract, and 64.7 g L-1 of MgCO3. Verification experiment indicated that the maximal succinic acid production of 52.7 ± 0.8 g L-1 was obtained under the identified optimal conditions. The result agreed with the predicted value well. Compared with that of the basic medium, the production of succinic acid and yield of succinic acid against glucose were enhanced by 67.3% and 111.1%, respectively. The results obtained in this study may be useful for the industrial commercial production of succinic acid. [ABSTRACT FROM AUTHOR]

Published
2012
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