Search

Your search keyword '"Han, Shuang"' showing total 23 results
Start Over Author "Han, Shuang" Publisher hindawi limited
23 results on '"Han, Shuang"'

7. 5-Aminosalicylic Acid Prevents Disease Behavior Progression and Intestinal Resection in Colonic and Ileocolonic Crohn’s Disease Patients: A Retrospective Study

Author

Wan, Jian, primary, Wang, Xuan, additional, Zhang, Yujie, additional, Xue, Xianmin, additional, Li, Yani, additional, Liu, Zhenzhen, additional, Han, Shuang, additional, Chen, Min, additional, Nie, Yan, additional, Shi, Yongquan, additional, Liang, Jie, additional, and Wu, Kaichun, additional

Published
2021
Full Text
View/download PDF

8. ATXN2-Mediated PI3K/AKT Activation Confers Gastric Cancer Chemoresistance and Attenuates CD8+ T Cell Cytotoxicity.

Author

Wang, Qi, Cao, Tianyu, Zhang, Xiaohui, Hui, Juan, Wang, Chen, Zhang, Wenyao, Wang, Pei, Zhou, Yun, and Han, Shuang

Subjects
STOMACH cancer, DRUG resistance in cancer cells, T cells, PI3K/AKT pathway, REPORTER genes
Abstract

As one of the primary therapeutic choices, chemotherapy is widely adopted for progressive gastric cancer (GC), but the development of chemoresistance has limited chemotherapy efficacy and partly contributes to poor prognosis. Immunotherapy is increasingly being applied in the clinical treatment of GC and is also benefitting patients. To ascertain whether ATXN2 affects chemotherapy efficacy in GC cells and its role in GC immune escape, we performed high-throughput sequencing to clarify genes differentially expressed between 5-FU-resistant and 5-FU-sensitive GC cells and then conducted qRT-PCR to assess ATXN2 expression in GC tissues. Furthermore, the influence of ATXN2 on resistance was studied in vitro and in vivo, ATXN2 and other protein expression levels were detected using Western blotting and immunohistochemistry (IHC), and the direct association of SP1 and ATXN2 was confirmed through luciferase reporter gene analysis. We found elevated ATXN2 in GC tumors and a negative correlation between ATXN2 levels and the prognosis of GC. Furthermore, by activating the PI3K/AKT pathway, ATXN2 was found to promote chemoresistance in GC, facilitating BCL2L1 expression. In GC cells, ATXN2 further stimulated PD-L1 expression and provided better immunotherapy efficacy. Finally, we demonstrated that SP1 transcriptionally regulated the expression of ATXN2 and prompted GC chemoresistance and immune escape. In conclusion, our study reveals the important roles of the SP1/ATXN2/PI3K-AKT/BCL2L1 signalling pathway in GC chemoresistance and of the SP1/ATXN2/PI3K-AKT/PD-L1 signalling pathway in GC immunotherapy. Our findings provide new theories and experimental references for overcoming chemotherapy resistance in GC and enhancing the efficacy of immunotherapy for GC. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

11. Development of a Chemiluminescence Immunoassay for Quantification of 25-Hydroxyvitamin D in Human Serum

Author

Zhonghu Bai, Wuxian Qiu, Han Shuang, Xiao Tong, and Zhang Junlan

Subjects
Vitamin, Streptavidin, Article Subject, General Chemical Engineering, Coefficient of variation, 030209 endocrinology & metabolism, 01 natural sciences, Horseradish peroxidase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Instrumentation, Thermostability, Carbodiimide, Chromatography, QD71-142, biology, Chemistry, 010401 analytical chemistry, 0104 chemical sciences, Computer Science Applications, Reagent, Biotinylation, biology.protein, Analytical chemistry, Research Article
Abstract

In this study, a chemiluminescence immunoassay (CLIA) for human serum 25-hydroxyvitamin D (25(OH)D) was established by a competition model. In serum, more than 99% of total circulating 25(OH)D binds to protein and less than 1% of 25(OH)D is in free form (Jassil et al., 2017). Before measuring concentration of 25(OH)D in serum, a releasing procedure should be conducted. A new reagent is used to release binding 25(OH)D to free form. Streptavidin (SA) was labeled to magnetic beads by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) method. Biotinylated VD was used as a competitor of 25(OH)D in samples. Anti-VD antibody (aby) was labeled to horseradish peroxidase (HRP) by EDC to react with 25(OH)D and biotinylated-VD molecules. The pretreated samples or standards were added into the reaction tube with biotin-VD and anti-VD aby-HRP, free 25(OH)D in the sample competes with biotinylated VD for binding to anti-VD aby-HRP, an SA-labeled magnetic particle is added to isolate the signal-generating complex, and the signal is inversely proportional to the 25(OH)D concentration in the sample. The method established shows good thermostability and performance. The limitation of detection (LoD) is 1.43 ng/mL. The intra-assay coefficient of variation (CV) is 3.66%–6.56%, the interassay CV is 4.19%–7.01%, and the recovery rate is 93.22%–107.99%. Cross-reactivity (CR) was remarkably low with vitamin D2, vitamin D3, 1, 25-dihydroxyvitamin D3, and 1, 25-dihydroxyvitamin D2. At the same time, the cross-reaction values with 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 were 97% and 100%, respectively. The developed method shows good correlation with the total VD product from Roche and DiaSorin. 1096 clinical patient samples were measured with developed reagent kit in this study. 7 types of disease were involved, and the concentration of 25(OH)D is less than 30 ng/mL in 94.98% of patients.

Published
2020

20. The Association between Bile Salt Export Pump Single-Nucleotide Polymorphisms and Primary Biliary Cirrhosis Susceptibility and Ursodeoxycholic Acid Response

Author

Chen, Rui-rui, primary, Li, Yuan-jun, additional, Zhou, Xin-min, additional, Wang, Lu, additional, Xing, Juan, additional, Han, Shuang, additional, Cui, Li-na, additional, Zheng, Lin-hua, additional, Wu, Kai-chun, additional, Shi, Yong-quan, additional, Han, Zhe-yi, additional, Han, Ying, additional, and Fan, Dai-ming, additional

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results