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Aims. This study examines mood and cardiovascular variables related to job stress and burnout in hospital personnel. Main Methods. 400 nurses and physicians from a children's hospital in China were recruited. Participants completed job stress, burnout, and mood state questionnaires. Cardiovascular variables such as body mass index (BMI), triglyceride (TG), and high density lipoprotein (HDL) were measured. Key Findings. Job stress and burnout were significantly associated with mood state. Statistically significant correlations were found between triglyceride levels and job stress scores (r = 0.175, P < 0.01), BMI and job stress scores (r = 0.121, P < 0.05), and HDL levels and job stress scores (r = -0.117, P < 0.05). Significance. Mood state changes may be related to job stress and job burnout, in turn, associated with triglycerides and HDL levels. Public health implications and interventions are discussed. [ABSTRACT FROM AUTHOR]