1. MicroRNA Expression in Salivary Supernatant of Patients with Pancreatic Cancer and Its Relationship with ZHENG
- Author
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Lianyu Chen, Peng Wang, Zhen Chen, Song Gao, and Lu Ming Liu
- Subjects
Adult ,Male ,Article Subject ,lcsh:Medicine ,Signs and symptoms ,Traditional Chinese medicine ,Bioinformatics ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,law ,Pancreatic cancer ,microRNA ,Humans ,Medicine ,Medicine, Chinese Traditional ,Salivary Proteins and Peptides ,Polymerase chain reaction ,Aged ,Regulation of gene expression ,General Immunology and Microbiology ,business.industry ,Gene Expression Profiling ,lcsh:R ,General Medicine ,Middle Aged ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Gene expression profiling ,MicroRNAs ,Real-time polymerase chain reaction ,Female ,business ,Research Article - Abstract
In traditional Chinese medicine (TCM), diagnosis and prescriptions are based on the signs and symptoms which are recognized as ZHENG. The cornerstone of TCM is to differentially treat one ZHENG from others, which is also known as syndrome differentiation, and this relies on the gathering of clinical information through inspection, auscultation and olfaction, inquiry, and palpation. However, the biomolecular basis of the ZHENG remains unclear. In this study, the expressions of 384 cancer-related miRNAs in salivary supernatant of patients with pancreatic cancer were assessed by miRNA polymerase chain reaction (PCR) array, and the different expression patterns of miRNA in three different groups of ZHENG were studied with use of real-time quantitative PCR (qRT-PCR). Some miRNAs were found to be specifically expressed in some ZHENGs, for instance, miR-17, miR-21, and miR-181b in Shi-Re ZHENG and miR-196a in Pi-Xu ZHENG. This indicates that these miRNAs may play important roles in different ZHENG condition. Therefore, this study to some extent revealed the molecular basis of TCM ZHENG in pancreatic cancer.
- Published
- 2014