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Your search keyword '"Raymond Simplice Mouokeu"' showing total 2 results
Start Over Author "Raymond Simplice Mouokeu" Publisher hindawi limited
2 results on '"Raymond Simplice Mouokeu"'

1. Influence of Processing Methods on Proximate Composition and Dieting of Two Amaranthus Species from West Cameroon

Author

Arnaud Landry Suffo Kamela, Raymond Simplice Mouokeu, Rawson Ashish, Ghislain Maffo Tazoho, Lamye Glory Moh, Etienne Pamo Tedonkeng, and Jules-Roger Kuiate

Subjects
Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456
Abstract

The effects of various processing methods on the proximate composition and dieting of Amaranthus hybridus and Amaranthus cruentus from West Cameroon were investigated in this study. Both amaranths leaves were subjected to same treatments (sun-dried and unsliced, sliced and cooked), milled, and analysed for their mineral and proximate composition. Thirty-Six Wistar albino rats of 21 to 24 days old were distributed in six groups and fed for 14 days with 10% protein based diets named D0 (protein-free diet), DI (egg white as reference protein), DII (sun-dried and unsliced A. hybridus), DIII (cooked and sliced A. hybridus), DIV (sun-dried and unsliced A. cruentus), and DV (cooked and sliced A. cruentus). The protein bioavailability and haematological and biochemical parameters were assessed in rats. The results showed that K, P, Mg, Zn, and Fe had the higher content in both samples regardless of processing method. The sun-dried and unsliced A. cruentus contained the highest value of crude protein 32.22 g/100 g DM (dry matter) while the highest crude lipid, 3.80 and 2.58%, was observed, respectively, in sun-dried and unsliced A. hybridus and cooked and sliced A. cruentus. Cooked and sliced A. hybridus and A. cruentus contained high crude fiber of 14 and 12.18%, respectively. Rats fed with diet DIII revealed the best protein bioavailability and haematological parameters whereas 100% mortality rate was recorded with group fed with diet DIV. From this study, it is evident that cooked and sliced A. hybridus and A. cruentus could play a role in weight reduction regimes.

Published
2016
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2. Effect of Fractioning on Antibacterial Activity ofEnantia chloranthaOliver (Annonaceae) Methanol Extract and Mode of Action

Author

Rosalie Anne Ngono Ngane, Cedric Laurel Cidjeu Pouaha, Jean Paul Assam Assam, François Xavier Etoa, Rebeca Madeleine Ebelle Etame, Igor Voukeng Kenfack, Raphael Tchientcheu, Jules-Roger Kuiate, Raymond Simplice Mouokeu, Alembert Tchinda Tiabou, and Frank Stève Monthe Poundeu

Subjects
0301 basic medicine, Minimum bactericidal concentration, Article Subject, Traditional medicine, biology, 010405 organic chemistry, Chemistry, Broth microdilution, lcsh:Other systems of medicine, Bacterial growth, lcsh:RZ201-999, biology.organism_classification, 01 natural sciences, 0104 chemical sciences, 03 medical and health sciences, 030104 developmental biology, Complementary and alternative medicine, Phytochemical, Maceration (wine), Antibacterial activity, Mode of action, Bacteria
Abstract

Infectious diseases caused by bacteria constitute the main cause of morbidity and mortality throughout the world and mainly in developing countries. In this work, the influence of fractioning and the mode of action of stem barks methanol extract ofEnantia chloranthawere investigated. The aim was to optimize the antibacterial activity of the methanol extract. The extract was prepared by maceration of barks powder in methanol. Fractioning was done using increasing solvents polarity. Standard phytochemical methods were used for phytochemical screening. Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) of the methanol extract and fractions were determined using broth microdilution method. The studied mode of action of both methanol extract and n-butanol fraction included antibiofilm activity, H+-ATPase-mediated proton pumping assay, salt tolerance, and cells cycle. The methanol extract ofE. chloranthastem barks was found to be active on all the bacteria tested (32 ≤ MIC ≤ 512 μg/mL), its activity being significant (MIC < 100 μg/ml) out of 5 of the 28 clinical isolates used.Salmonella entericaserovar paratyphi A was the most sensitive (32 μg/mL). Compared to the extract and other fractions, the n-butanol fraction was found to be more active (32 ≤ MIC ≤ 256). Significant antibacterial activity of this fraction was observed out of 10 of the 28 bacterial isolates and 3 out of 7 bacterial strains. Lowest MIC values (32 μg/ml) of this fraction were obtained withEscherichia coli(136),Pseudomonas aeruginosa(CIP 76110), andSalmonella entericaserovar typhi 9. The methanol extract ofE. chloranthaand its n-butanol fraction revealed several modes of action including the prolongation of the latency phase of the bacterial growth, the inhibition of the pump with protons H+- ATPases bacterial, the loss of the salt tolerance of theStaphylococcus aureus,and inhibition of the formation of the bacterial biofilm. The present results showed that the n-butanol fraction of the methanol stem barks extract ofE. chloranthapossess the essential antibacterial components and could best be used to fight against bacterial infections as compared to methanol extract.

Published
2018
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