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Your search keyword '"Zhao, Yizhou"' showing total 8 results
Start Over Author "Zhao, Yizhou" Publisher hindawi limited
8 results on '"Zhao, Yizhou"'

5. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway.

Author

Wu, Shuang, Yang, Tiantian, Cen, Kai, Zou, Yihuai, Shi, Xiaowei, Zhou, Dongrui, Gao, Yonghong, Chai, Limin, Zhao, Yizhou, Sun, Yikun, and Zhu, Lingqun

Subjects
APOPTOSIS, CELL receptors, CELLULAR signal transduction, CEREBRAL ischemia, EPIDERMAL growth factor, GENE expression, GLYCOSIDES, HERBAL medicine, LACTATE dehydrogenase, CHINESE medicine, MESSENGER RNA, MOLECULAR structure, PHOSPHORYLATION, POLYMERASE chain reaction, PROTEIN kinases, WESTERN immunoblotting, CELL survival, IN vitro studies, DRUG administration, DRUG dosage
Abstract

Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells. [ABSTRACT FROM AUTHOR]

Published
2020
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6. Yiqihuoxuejiedu Formula Restrains Vascular Remodeling by Reducing the Inflammation Reaction and Cx43 Expression in the Adventitia after Balloon Injury

Author

Chang, Hong, primary, Lei, Huan, additional, Zhao, Yizhou, additional, Yang, Ruixue, additional, Wu, Aiming, additional, Mao, Yingqiu, additional, Huang, Youliang, additional, Lv, Xiying, additional, Zhao, Jiuli, additional, Lou, Lixia, additional, Zhang, Dongmei, additional, He, Yingkun, additional, Xu, Ying, additional, Yang, Tao, additional, and Zhao, Mingjing, additional

Published
2015
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