1. Noncoding RNAs: Possible Players in the Development of Fluorosis
- Author
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Devendra Parmar, Pravin K. Naoghare, Patrizio Arrigo, Hemant J. Purohit, Amit Bafana, Saravanadevi Sivanesan, Krishnamurthi Kannan, Alberto Izzotti, and Atul P. Daiwile
- Subjects
Genetics and Molecular Biology (all) ,Fluorosis, Dental ,Article Subject ,Cell Survival ,Immunology and Microbiology (all) ,lcsh:Medicine ,Environmental pollution ,Core Binding Factor Alpha 1 Subunit ,Biology ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,chemistry.chemical_compound ,Fluorosis ,Cell Line, Tumor ,microRNA ,Sodium fluoride ,Transcriptional regulation ,RNA, Small Nucleolar ,Humans ,Epigenetics ,Small nucleolar RNA ,Transcription factor ,Small Nucleolar ,Regulation of gene expression ,Cell Differentiation ,Gene Expression Regulation ,MicroRNAs ,Osteosarcoma ,RANK Ligand ,Signal Transduction ,Sodium Fluoride ,Biochemistry, Genetics and Molecular Biology (all) ,Tumor ,environmental genomics ,General Immunology and Microbiology ,lcsh:R ,General Medicine ,Molecular biology ,ncRNA ,chemistry ,Dental ,RNA ,Research Article - Abstract
Fluorosis is caused by excess of fluoride intake over a long period of time. Aberrant change in the Runt-related transcription factor 2 (RUNX2) mediated signaling cascade is one of the decisive steps during the pathogenesis of fluorosis. Up to date, role of fluoride on the epigenetic alterations is not studied. In the present study, global expression profiling of short noncoding RNAs, in particular miRNAs and snoRNAs, was carried out in sodium fluoride (NaF) treated human osteosarcoma (HOS) cells to understand their possible role in the development of fluorosis. qPCR and in silico hybridization revealed that miR-124 and miR-155 can be directly involved in the transcriptional regulation of Runt-related transcription factor 2 (RUNX2) and receptor activator of nuclear factorκ-B ligand (RANKL) genes. Compared to control, C/D box analysis revealed marked elevation in the number of UG dinucleotides and D-box sequences in NaF exposed HOS cells. Herein, we report miR-124 and miR-155 as the new possible players involved in the development of fluorosis. We show that the alterations in UG dinucleotides and D-box sequences of snoRNAs could be due to NaF exposure.
- Published
- 2015