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Your search keyword '"Guedes, Liane"' showing total 3 results
Start Over Author "Guedes, Liane" Publisher hindawi publishing corporation
3 results on '"Guedes, Liane"'

2. Modifying graft-versus-host disease in a humanized mouse model by targeting macrophages or b-cells

Author

Pathologie Moleculair Path., Pathologie Pathologen staf, Cancer, Pathologie Laboratorium diagnostiek, Circulatory Health, Hogenes, Marieke C.H., Van Dorp, Suzanne, Van Kuik, Joyce, Monteiro, Filipa R.P., Ter Hoeve, Natalie, Guedes, Liane, Van Dijk, Marijke R., Martens, Anton C., De Weger, Roel A., Pathologie Moleculair Path., Pathologie Pathologen staf, Cancer, Pathologie Laboratorium diagnostiek, Circulatory Health, Hogenes, Marieke C.H., Van Dorp, Suzanne, Van Kuik, Joyce, Monteiro, Filipa R.P., Ter Hoeve, Natalie, Guedes, Liane, Van Dijk, Marijke R., Martens, Anton C., and De Weger, Roel A.

Published
2019

3. Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells.

Author

Hogenes MCH, van Dorp S, van Kuik J, Monteiro FRP, Ter Hoeve N, Guedes L, van Dijk MR, Martens AC, and de Weger RA

Subjects
Animals, Disease Models, Animal, Female, Humans, Lymphocyte Depletion, Mice, Mice, SCID, B-Lymphocytes immunology, Graft vs Host Disease immunology, Hematopoietic Stem Cell Transplantation, Leukocytes, Mononuclear immunology, Liver Cirrhosis immunology, Macrophages immunology, T-Lymphocytes, Regulatory immunology
Abstract

Humanized mouse models can well be modified to study specific aspects of Graft-versus-Host Disease (GvHD). This paper shows the results of both macrophage depletion and (early) B-cell depletion in a humanized mouse model using RAG2 -/- γ c -/- mice injected with HuPBMCs. Macrophage depletion showed a significant decrease in survival and also lead to a change in the histomorphology of the xenogeneic reaction. Higher levels of infiltrating B-cells were observed in various organs of mice depleted for macrophages. With (early) B-cell depletion using Rituximab, a clear improvement on clinical symptoms was observed, even when probably only inactivated B-cells were deleted. However, the histological examinations only showed a significant morphological effect on liver fibrosis. This may be related to a difference in the mRNA levels of TGF- β . Also, lower mRNA levels of Tregs in some organs were observed after Rituximab treatment, which contradicts that a higher number of Tregs would always be related to less severe GvHD. Our data show that both macrophage depletion and (early) B-cell depletion in a xenogeneic mouse model can influence the clinical, histological, and cytokine production of a GvHD response.

Published
2019
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