1. Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
- Author
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Gilberto U.L. Braga, Andréia Ávila Soares de Oliveira, Marilesia Ferreira de Souza, Marcelo Tempesta de Oliveira, Ilce Mara de Syllos Cólus, Gustavo Rafael Mazzaron Barcelos, Fernando Barbosa, Rossana Batista de Oliveira Godoy Camargo, and André van Helvoort Lengert
- Subjects
medicine.medical_specialty ,Meat ,Genotype ,Article Subject ,Population ,lcsh:Medicine ,Biology ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,GSTP1 ,Gene Frequency ,Internal medicine ,medicine ,Animals ,Humans ,education ,Allele frequency ,Methylmercury ,Life Style ,Whole blood ,Genetics ,education.field_of_study ,Polymorphism, Genetic ,General Immunology and Microbiology ,GCLM ,lcsh:R ,Fishes ,General Medicine ,Glutathione ,Feeding Behavior ,Mercury ,Methylmercury Compounds ,Endocrinology ,GCLC ,chemistry ,Multivariate Analysis ,Biomarkers ,Brazil ,Research Article - Abstract
This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1,GSTT1,GSTP1,GCLM, andGCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n=88), exposed to MeHg from fish consumption, were enrolled in the study. Hg species in the plasma compartment were determined by LC-ICP-MS, whereas genotyping was performed by PCR assays. Mean total Hg levels in plasma (THgP) and whole blood (THgB) were10±4.2and37±21, whereas mean evels of plasmatic MeHg (MeHgP), inorganic Hg (IHgP), and HgP/HgB were4.3±2.9,5.8±2.3 µg/L, and0.33±0.15, respectively.GSTM1andGCLCpolymorphisms influence THgP and MeHgP (multivariate analyses,P<0.050). Null homozygotes forGSTM1showed higher THgP and MeHgP levels compared to subjects withGSTM1(THgPβ=0.22,P=0.035; MeHgPβ=0.30,P=0.050) and persons carrying at least one T allele forGCLChad significant higher MeHgP (β=0.59,P=0.046). Also, polymorphicGCLMsubjects had lower THgP/THgB than those with the nonvariant genotype. Taken together, data of this study suggest that GSH-related polymorphisms may change the metabolism of MeHg by modifying the distribution of mercury species iin plasma compartment and the HgP/HgB partitioning.
- Published
- 2014
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