1. In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus
- Author
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Manel Amri, Dalila Mezioug, Razika Zeghir-Bouteldja, Saliha Ait-Aissa, Samia Bouaziz, and Chafia Touil-Boukoffa
- Subjects
inorganic chemicals ,Pathology ,medicine.medical_specialty ,biology ,Article Subject ,biology.organism_classification ,medicine.disease ,Molecular biology ,Echinococcosis ,In vitro ,Nitric oxide ,lcsh:Infectious and parasitic diseases ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,In vivo ,parasitic diseases ,medicine ,Cytotoxic T cell ,Parasitology ,lcsh:RC109-216 ,Nitrite ,Echinococcus granulosus ,Peroxynitrite ,Research Article - Abstract
Hydatidosis is characterized by the long-term coexistence of larvaEchinococcus granulosusand its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity ofNO2−andONOO−24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated thatONOO−is more cytotoxic thanNO2−. In contrast,NO3−has no effect. These results suggest possible involvement ofNO2−andONOO−in antihydatic action and point the efficacy of these metabolites as scolicidal agents.
- Published
- 2009