1. Biolistics-based gene silencing in plants using a modified particle inflow gun.
- Author
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Davies KM, Deroles SC, Boase MR, Hunter DA, and Schwinn KE
- Subjects
- Antirrhinum enzymology, Antirrhinum growth & development, Antirrhinum metabolism, Cells, Cultured, Culture Techniques, Cytochrome P-450 Enzyme System deficiency, Cytochrome P-450 Enzyme System genetics, DNA administration & dosage, DNA chemistry, DNA genetics, Flowers growth & development, Gold chemistry, Inverted Repeat Sequences genetics, Phenotype, Pigmentation genetics, Plant Proteins genetics, Solanum tuberosum cytology, Transcription Factors genetics, Transformation, Genetic, Antirrhinum genetics, Biolistics instrumentation, RNA Interference, Solanum tuberosum genetics
- Abstract
RNA interference (RNAi) is one of the most commonly used techniques for examining the function of genes of interest. In this chapter we present two examples of RNAi that use the particle inflow gun for delivery of the DNA constructs. In one example transient RNAi is used to show the function of an anthocyanin regulatory gene in flower petals. In the second example stably transformed cell cultures are produced with an RNAi construct that results in a change in the anthocyanin hydroxylation pattern.
- Published
- 2013
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