1. Inhibition of the SUMO Pathway by Gam1
- Author
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Susanna Chiocca, Mariaelena Pozzebon, and Chiara V. Segré
- Subjects
enzymes and coenzymes (carbohydrates) ,In vivo ,Chemistry ,genetic processes ,health occupations ,SUMO protein ,SUMO enzymes ,macromolecular substances ,environment and public health ,In vitro ,Cell biology - Abstract
We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.
- Published
- 2009
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