1. Effects of exogenous zinc on cell cycle, apoptosis and viability of MDAMB231, HepG2 and 293 T cells.
- Author
-
Wang YH, Li KJ, Mao L, Hu X, Zhao WJ, Hu A, Lian HZ, and Zheng WJ
- Subjects
- Apoptosis genetics, Cation Transport Proteins genetics, Cell Cycle genetics, Cell Line, Tumor, Cell Survival drug effects, Cell Survival genetics, Dose-Response Relationship, Drug, Flow Cytometry, G1 Phase Cell Cycle Checkpoints drug effects, G1 Phase Cell Cycle Checkpoints genetics, G2 Phase Cell Cycle Checkpoints drug effects, G2 Phase Cell Cycle Checkpoints genetics, Gene Expression drug effects, HEK293 Cells, Hep G2 Cells, Humans, Microscopy, Confocal, Protein Isoforms genetics, Reverse Transcriptase Polymerase Chain Reaction, Zinc metabolism, Zinc Sulfate metabolism, Zinc Sulfate pharmacology, Apoptosis drug effects, Cell Cycle drug effects, Zinc pharmacology
- Abstract
As a non-toxic metal to humans, zinc is essential for cell proliferation, differentiation, regulation of DNA synthesis, genomic stability and mitosis. Zinc homeostasis in cells, which is crucial for normal cellular functioning, is maintained by various protein families including ZnT (zinc transporter/SLC30A) and ZIP (Zrt-, Irt-like proteins/SLC39A) that decrease and increase cytosolic zinc availability, respectively. In this study, we investigated the influences of a specific concentration range of ZnSO4 on cell cycle and apoptosis by flow cytometry, and cell viability by MTT method in MDAMB231, HepG2 and 293 T cell lines. Fluorescent sensors NBD-TPEA and the counterstain for nuclei Hoechst 33342 were used to stain the treated cells for observing the localisation and amount of Zn(2+) via laser scanning confocal microscope. It was found that the influence manners of ZnSO4 on cell cycle, apoptosis and cell viability in various cell lines were different and corresponding to the changes of Zn(2+) content of the three cell lines, respectively. The significant increase on intracelluar zinc content of MDAMB231 cells resulted in cell death, G1 and G2/M cell cycle arrest and increased apoptotic fraction. Additionally, the mRNA expression levels of ZnT and ZIP families in the three cell lines, when treated with high concentration of ZnSO4, increased and decreased corresponding to their functions, respectively.
- Published
- 2013
- Full Text
- View/download PDF