1. Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells.
- Author
-
Vidoni C, Ferraresi A, Seca C, Secomandi E, and Isidoro C
- Subjects
- Animals, Autophagosomes drug effects, Autophagosomes pathology, Autophagy-Related Proteins metabolism, Carcinogenesis pathology, Cell Culture Techniques instrumentation, Cell Culture Techniques methods, Cell Line, Tumor, Chloroquine pharmacology, Disease Progression, Electrophoresis, Polyacrylamide Gel instrumentation, Electrophoresis, Polyacrylamide Gel methods, Fluorescent Dyes chemistry, Humans, Immunoblotting instrumentation, Lysosomes pathology, Macrolides pharmacology, Mice, Microscopy, Fluorescence instrumentation, Microscopy, Fluorescence methods, Pancreas cytology, Pancreas pathology, Autophagy, Autophagy-Related Proteins analysis, Immunoblotting methods, Pancreatic Neoplasms pathology
- Abstract
Macroautophagy is a catabolic process through which redundant, aged, or damaged cellular structures are first enclosed within double-membrane vesicles (called autophagosomes), and thereafter degraded within lysosomes. Macroautophagy provides a primary route for the turnover of macromolecules, membranes and organelles, and as such plays a major role in cell homeostasis. As part of the stress response, autophagy is crucial to determine the cell fate in response to extracellular or intracellular injuries. Autophagy is involved in cancerogenesis and in cancer progression. Here we illustrate the essential methods for monitoring autophagy in pancreatic cancer cells.
- Published
- 2019
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