1. Somatic Embryogenesis in Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae).
- Author
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Guerra MP, Steiner N, Farias-Soares FL, Vieira Ldo N, Fraga HP, Rogge-Renner GD, and Maldonado SB
- Subjects
- Brazil, Flowers genetics, Flowers growth & development, Germination genetics, Seeds genetics, Seeds growth & development, Tracheophyta genetics, Plant Development genetics, Plant Somatic Embryogenesis Techniques methods, Tissue Culture Techniques methods, Tracheophyta growth & development
- Abstract
This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan's blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages.
- Published
- 2016
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