1. Immunophenotypic Analysis of CAR-T Cells.
- Author
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de Azevedo JTC, Mizukami A, Moço PD, and Malmegrim KCR
- Subjects
- Animals, Biomarkers, Cytotoxicity, Immunologic, Flow Cytometry, Humans, Immunologic Memory, Immunotherapy, Adoptive methods, Immunotherapy, Adoptive standards, Lymphocyte Activation, Mice, Receptors, Antigen, T-Cell genetics, Receptors, Chimeric Antigen genetics, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, T-Lymphocytes immunology, Transduction, Genetic, Immunophenotyping methods, Receptors, Antigen, T-Cell metabolism, Receptors, Chimeric Antigen metabolism, T-Lymphocytes metabolism
- Abstract
CAR-T cell immunotherapy is a promising therapeutic modality for cancer patients. The success of CAR-T cell therapy has been associated with the phenotype, activation and functional profiling of infused CAR-T cells. Therefore, immunophenotypic characterization of CAR-T cells during bioprocess is crucial for cell quality control and ultimately for improved antitumor efficacy. In this chapter, we propose a flow cytometry panel to characterize the immunophenotype of the CAR-T subsets.
- Published
- 2020
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