1. Phospho-Flow Analysis of Primary Mouse Cells After HDAC Inhibitor Treatment.
- Author
-
Hildebrand D and Kubatzky KF
- Subjects
- Animals, Antibodies, Monoclonal chemistry, Bone Marrow Cells cytology, Bone Marrow Cells metabolism, Cell Separation methods, Fluorescein-5-isothiocyanate chemistry, Gene Expression, Histone Deacetylases genetics, Histone Deacetylases metabolism, Interferon-gamma pharmacology, Mice, Mice, Inbred C57BL, Phosphorylation, Primary Cell Culture, STAT1 Transcription Factor genetics, STAT1 Transcription Factor metabolism, Benzamides pharmacology, Bone Marrow Cells drug effects, Flow Cytometry methods, Histone Deacetylase Inhibitors pharmacology, Pyridines pharmacology, Staining and Labeling methods
- Abstract
Flow cytometric techniques allow fast, sensitive, and multiparametric analyses at the single cell level. This makes it possible to distinguish subsets of cells within heterogeneous samples. Moreover, flow cytometry has become a frequently used method for the evaluation of therapeutic effects. Here, we describe the analysis of the phosphorylation status of signal transducer and activator of transcription-1 (STAT-1) in primary mouse cells after treatment with histone deacetylase inhibitors (HDACi) that are currently considered anticancer agents. We provide detailed protocols for the preparation of murine bone marrow cells and the staining of HDACi-treated cells, as well as an insight into the concepts of flow cytometry analysis.
- Published
- 2017
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