1. Culture of rat mesencephalic dopaminergic neurons and application to neurotoxic and neuroprotective agents
- Author
-
Massimo Barbierato, Morena Zusso, Laura Facci, Vanni Ferrari, and Stephen D. Skaper
- Subjects
0301 basic medicine ,1-Methyl-4-phenylpyridinium ,2,4,5-trihydroxyphenylalanine ,Brain-derived neurotrophic factor ,Dopamine uptake ,Mesencephalic ,Parkinson disease ,Rat ,Tyrosine hydroxylase ,Ventral tegmentum ,Molecular Biology ,Genetics ,Biology ,Neuroprotection ,03 medical and health sciences ,0302 clinical medicine ,Neurotrophic factors ,Dopaminergic Cell ,Neurotoxin ,Dopaminergic ,030104 developmental biology ,Neuroscience ,030217 neurology & neurosurgery ,5-trihydroxyphenylalanine - Abstract
Dopaminergic neuronal cell degeneration is the principal characteristic feature of the neuropathology of Parkinson disease. Cultures of mesencephalic neurons are widely used as a source of dopaminergic neurons for the study of mechanisms implicated in dopaminergic cell death and for the evaluation of potential dopaminergic neuroprotective agents, including neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat mesencephalic cell cultures and their application to evaluating the effect of the dopaminergic neurotoxin 1-methyl-4-phenylpyridinium and the neuroprotective action of brain-derived neurotrophic factor.
- Published
- 2018