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We report on a novel approach to monitor photodynamic therapy procedure based on the simultaneous fluorescence and optoacoustic imaging with the use of a liposome nanoconstructs contain benzoporphyrin derivatives and the fluorescent IRDye800 dye. We demonstrate the results of a preliminary in vivo study with combined fluorescence and optoacoustic custom-made setups on a NUDE mouse with human glioblastoma U-87.

The fluorescence imaging device for localizing a tumor in a patient's body and monitoring photobleaching of a photosensitizer (PS) in course of photodynamic therapy was constructed. The system includes two LED sources at 405 and 660 nm for excitation of chlorine-based PSs. The ratio of fluorescence signals upon excitation at these wavelengths was demonstrated to provide evaluation of PS penetration depth in biotissues after topical administration. The study included Monte Carlo simulations that are in a good agreement with phantom experiment.

The paper reviews our recent experience in multiscale biomedical optoacoustic imaging using wideband ultrasonic detectors based on polyvinylidene fluoride films. The experimental setups for optoacoustic microscopy and tomography are presented, numerical algorithms for versatile characterization of tissue vasculature are discussed.