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// Qingbin Zhao 1 , Huiyi Wei 1 , Dandan Liu 2 , Baolan Shi 3 , Lei Li 3 , Mengdan Yan 4 , Xiyang Zhang 5 , Fengjiao Wang 5 , Yongri Ouyang 4 1 Department of Geratology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, China 2 Department of Endocrinology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, China 3 Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010050, China 4 School of Life Science, Northwest University, Xi’an, Shaanxi, 710069, China 5 Xi’an Tiangen Precision Medical Institute, Xi’an, Shaanxi 710075, China Correspondence to: Qingbin Zhao, email: zhaoqingbin8244693@163.com Keywords: coronary artery disease (CAD), single nucleotide polymorphism (SNP), PHACTR1, SLC22A3, case-control study Received: September 22, 2016 Accepted: November 12, 2016 Published: November 22, 2016 ABSTRACT Previous studies showed that PHACTR1 and SLC22A3 are involved in coronary vascular development and are key determinants of cardiovascular disease risk. We conducted a case-control study to examine the effect of SLC22A3 and PHACTR1 single nucleotide polymorphisms (SNPs) on CAD risk among 376 male CAD patients and 388 male healthy controls from China. Eleven SLC22A3 and PHACTR1 SNPs were selected and genotyped using Sequenom Mass-ARRAY technology. Odds ratios (OR) and 95% confidence intervals (CIs) were calculated using unconditional logistic regression adjusting for age. The rs9381439 minor allele “A” (OR = 0.72; 95% CI = 0.54–0.96; p = 0.024) in an allelic model was associated with reduced CAD risk, as were the rs2048327 “C/C” (OR = 0.60; 95% CI: 0.37–0.97; p = 0.036) and rs1810126 “T/T” (OR = 0.58; 95% CI: 0.36–0.93; p = 0.024) genotypes. Likewise, the rs9349379 “A/G” genotype in a dominant model ( p = 0.041), the rs1810126 “T/C” genotype in additive ( p = 0.041) and recessive ( p = 0.012) models, and the rs2048327 “C/T” genotype in a recessive model were associated with decreased CAD risk ( p = 0.016). These results suggest several PHACTR1 and SLC22A3 polymorphisms are associated with decreased CAD risk in the male Chinese Han population.

// Qingbin Zhao 1 , Shudan Liao 2 , Huiyi Wei 1 , Dandan Liu 3 , Jingjie Li 4,5 , Xiyang Zhang 5 , Mengdan Yan 4,5 and Tianbo Jin 4,5 1 Department of Geratology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China 2 Department of Cardiology, Xi’an Center Hospital, Xi’an, Shaanxi, China 3 Department of Endocrinology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China 4 Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi’an, Shaanxi, China 5 Xi’an Tiangen Precision Medical Institute, Xi’an, Shaanxi, China Correspondence to: Qingbin Zhao, email: // Keywords : coronary heart disease; single nucleotide polymorphisms; CDKN2BAS; case-control,Gerotarget Received : September 01, 2016 Accepted : October 05, 2016 Published : October 11, 2016 Abstract The goal of our study was to determine whether CDKN2BAS polymorphisms are associated with coronary heart disease (CHD) risk in a Han Chinese population. Eight SNPs were genotyped in 676 men and 465 women. We used χ 2 tests and genetic model analyses to evaluate associations between the SNPs and CHD risk. We found that rs10757274 was associated with an increased risk of CHD in both men (allele G: Odds ratio [OR] = 1.30, 95% confidence interval [CI]: 1.05-1.61, P = 0.018; codominant model: P = 0.042; recessive model: OR = 1.70, 95% CI: 1.10-2.62, P = 0.016; log-additive model: OR = 1.34, 95% CI: 1.05-1.71, P = 0.019) and women (dominant model: OR = 2.26, 95% CI: 1.28-3.99, P = 0.004). In addition, rs7865618 was associated with an 8.10-fold increased risk of CHD in women under a recessive model (OR = 8.10, 95% CI: 1.74-37.68, P = 0.006). Interestingly, the haplotype AA (rs10757274 and rs1333042) of CDKN2BAS was associated with decreased the risk of CHD in men (OR = 0.72, 95% CI: 0.55 - 0.95, P = 0.022).