1. Improved muscle function and quality after diet intervention with leucine-enriched whey and antioxidants in antioxidant deficient aged mice
- Author
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Yvette Luiking, Stéphane Walrand, Sjors Verlaan, Klaske van Norren, Francina J. Dijk, Dorien A.M. van Dartel, Annelies Bunschoten, Miriam van Dijk, Marion Jourdan, Nutricia Research, Wageningen University and Research Centre (WUR), Unité de Nutrition Humaine (UNH), and Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA)
- Subjects
Male ,0301 basic medicine ,Sarcopenia ,Whey protein ,Antioxidant ,medicine.medical_treatment ,Skeletal muscle ,medicine.disease_cause ,Antioxidants ,Mice ,Random Allocation ,chemistry.chemical_compound ,Research Paper: Gerotarget (Focus on Aging) ,Casein ,Medicine ,Fatigue ,ComputingMilieux_MISCELLANEOUS ,2. Zero hunger ,Gerotarget ,Age Factors ,Nutritional Biology ,medicine.anatomical_structure ,Oncology ,Biochemistry ,sarcopenia ,skeletal muscle ,fatigue ,antioxidants ,Human and Animal Physiology ,Alimentation et Nutrition ,Models, Animal ,Vitamin ,medicine.medical_specialty ,03 medical and health sciences ,antioxydant ,sarcopenie ,Leucine ,Internal medicine ,Food and Nutrition ,Animals ,Muscle, Skeletal ,VLAG ,030109 nutrition & dietetics ,Muscle fatigue ,business.industry ,stress oxydatif ,medicine.disease ,Diet ,Mice, Inbred C57BL ,Oxidative Stress ,Whey Proteins ,030104 developmental biology ,Endocrinology ,chemistry ,Fysiologie van Mens en Dier ,business ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Oxidative stress - Abstract
Antioxidant (AOX) deficiencies are commonly observed in older adults and oxidative stress has been suggested to contribute to sarcopenia. Here we investigate if 1) low levels of dietary antioxidants had a negative impact on parameters of muscle mass, function and quality, and 2) to study if nutritional interventions with AOX and/or leucine-enriched whey protein could improve these muscle parameters in aged mice. 18-months-old mice were fed a casein-based antioxidant-deficient (lowox) diet or a casein-based control-diet (CTRL) for 7 months. During the last 3 months, lowox-mice were subjected to either: a) continued lowox, b) supplementation with vitamin A/E, Selenium and Zinc (AOX), c) substitution of casein with leucine-enriched whey protein (PROT) or d) a combination of both AOX and PROT (TOTAL). After 7 months lowox-mice displayed lower muscle strength and more muscle fatigue compared to CTRL. Compared to lowox-mice, PROT-mice showed improved muscle power, grip strength and less muscle fatigue. AOX-mice showed improved oxidative status, less muscle fatigue, improved grip strength and mitochondrial dynamics compared to lowox-mice. The TOTAL-mice showed the combined effects of both interventions compared to lowox-mice. In conclusion, nutritional intervention with AOX and/or leucine-enriched whey protein can play a role in improving muscle health in a AOX-deficient mouse model.
- Published
- 2016
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