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Background: Ovarian torsion is an important gynecological emergency and various agents are used in the experimental phase to prevent ovarian injury. The aim of this study was to determine the effect of granulocyte-colony-stimulating factor (G-CSF) use on ischemia/reperfusion injury in torsion/detorsion model of rat ovary. Methods: Twenty-eight Wistar-albino rats were randomly divided into four groups. The first group was designated as the sham group, and torsion/detorsion was applied to the remaining three groups. The third group was given saline and the fourth group was given G-CSF before detorsion. The total antioxidant and oxidant status, oxidative stress index, catalase, malondialdehyde and total thiol values were measured in rat ovaries, and after torsion/detorsion the follicles numbers of rat ovary were determined by histopathological examination. Results: There was no significant difference between groups in oxidative stress markers. However, the primary and secondary follicle numbers in the G-CSF group were significantly higher than the other torsion/detorsion groups (p < 0.01). Conclusions: Although the use of G-CSF in the rat ovary torsion/detorsion model was histopathologically protective in terms of the number of primary and secondary follicles, no difference was found in biochemical markers associated with reperfusion injury.