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Tuberculosis is the most ancient and resistant entity in the world of microbes that holds significant attention due to its substantial organ association. A lot has been done in diagnosing it and much is desired to isolate and enfold it in order to eradicate it especially from the third world countries. Presenting as a pulmonary inhabitant, it translocates itself in every organ causing significant morbidity and mortality. Recently, advances have been made in the field of molecular diagnostics to markedly improve the accuracy and point-in-time diagnosis. In addition, more promising methods are under development to further optimize the diagnosis.