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Species differences are known to exist with reference to LDH profile. A four-band isoenzyme pattern is a feature in most snakes, which is a deviation from the primitive ancestor. The LDH morphic state of two water snakes, Cerberus rhynchops and Xenochrophis piscator belonging to the same family Colubridae was selected for the geneological understanding since one of the species is a known salt tolerant. The study was undertaken to observe the fractions of the key isoenzyme LDH subunit diversity in an evolutionary backdrop.