101. Biomineralization of 2,3,4,6-Tetrachlorophenol byBacillussp. andStaphylococcussp. Isolated from Secondary Sludge of Pulp and Paper Mill
- Author
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Santosh Kumar Karn, M. S. Reddy, and Swapan Kumar Chakrabarti
- Subjects
biology ,business.industry ,Pulp (paper) ,Paper mill ,engineering.material ,medicine.disease_cause ,biology.organism_classification ,16S ribosomal RNA ,Chloride ,High-performance liquid chromatography ,Microbiology ,medicine ,engineering ,Food science ,business ,Staphylococcus ,Bacteria ,General Environmental Science ,Bacillus megaterium ,medicine.drug - Abstract
Three 2,3,4,6-tetrachlorophenol (2,3,4,6-TeCP)-mineralizing bacteria were isolated from the secondary sludge of a pulp and paper industry. The isolates used 2,3,4,6-TeCP as a source of carbon and energy and were capable of degrading this compound, as indicated by stoichiometric release of chloride and biomass formation. Based on 16S rRNA gene sequence analysis, the bacteria were identified as Bacillus megaterium (CL3), Staphylococcus suciri (CL10), and Bacillus thuringensis (CL11). High-performance liquid chromatography (HPLC) analysis revealed that these isolates were able to degrade 2,3,4,6-TeCP at higher concentrations (600 mg/L or 2.5 mM). A consortia of the isolates completely removed 2,3,4,6-TeCP from the sludge obtained from a pulp and paper mill within 2 weeks when supplemented at a rate of 100 mg/L or 0.43 mM. A bacterial consortium also significantly reduced absorbable organic halogen (AOX) and extractable organic halogen (EOX) by 63% and 68%, respectively, from the sludge. These isolate...
- Published
- 2014