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Strawberry (Fragaria ananassa Duch.) is one of the most important berries in the world. Amplified fragment length polymorphism (AFLP) was used to evaluate the genetic diversity of strawberry in Motuo County of the Tibet Plateau. Seventy strawberry samples grown in Motuo County were collected for AFLP analysis using 256 primer combinations. A set of 10 primer combinations produced 374 clearly identifiable AFLP loci. The polymorphism ranged from 50% to 64% with an average of 58.8%. Nei's genetic differentiation index for AFLP was 0.75, and the estimate of gene flow was less than 1 (Nm, 0.41). The unweighted pair group method with arithmetic mean dendrogram and Bayesian analysis clustered 70 samples into six groups. These findings clearly indicated high genetic diversity in wild strawberry from the Motuo County. These results could lay the foundation for further breeding and conservation of strawberry.

SummaryClonal selection is an important method for varietal improvement in grapevine. Ampelometric and morphological markers fail to differentiate clones from their parent genotype. Molecular markers offer the opportunity to identify the clonal material. In this study, five clones of the grapevine variety ‘Kishmish Chernyi’ were analysed using microsatellite (SSR) and AFLP markers. These clones differed significantly in their bunch characteristics including berry size, shape, and colour. Microsatellite (SSR) analysis using 24 primers could not distinguish between these clones. The allele profiles of the clones and the parent variety were identical. AFLP analysis using 13 primer pair combinations yielded 592 markers ranging in size from 50 – 500 bp. Of these, 79 markers (13%) were polymorphic. The majority of the polymorphic markers (75/79) were detected in the clone ‘Sharad Seedless’. Three AFLP primer combinations detected unique markers in three clones which could be useful for future identification.

SummaryHigh-throughput amplified fragment length polymorphism (AFLP) analysis, employing a two-dye automated DNA sequencer, was used to genotype accessions of four important Prunus spp. fruit crops in China, fruiting-mei (P. mume), apricot (P. armeniaca), plum (P. salicina, and P. ussuriensis) and peach (P. persica). Nine primer pairs generated a total of 2,089 AFLP bands, of which 94.4% were polymorphic. Levels of polymorphism and the genetic relationships among the crops were studied.The fixation index (Fst) of the four different fruit trees ranged from 0.53 – 0.82, and the average genetic diversity (AGD) in these species ranged from 3.2% to 12.8%. These four stone fruit species could be clustered into four clear groups on a phylogenetic tree based on bootstrap analysis. Phylogenetically, fruiting-mei was shown to be closest to apricot and most distant from peach.

Table grape genotypes under the ‘Amasya’ group are one of the widely grown table grape cultivars in many parts of Turkey. Although these genotypes are listed under same group. They had not been identified as same or different and it has caused problems in cultivar standardization and determination of total genotype number. This research was carried out to identfy Amasya genotypes belonging to the Amasya group using AFLP technique in DNA analysis. A total of 524 bands were obtained from 9 primer/enzyme combinations and 33.7% of them were found polymorphic. As a result of the study, all Amasya genotypes were declared to be a different cultivar. EcoRI +2 bases primer combinations were proved to be the most effective ones in polymorphic identifications in AFLP analysis.

SummaryThe success of pepino (Solanum muricatum), a vegetatively propagated crop, as an alternative for vegetable crop diversi®cation requires the development of new cultivars that combine both high yield and good fruit quality. Four unrelated clones, complementary for yield (Sm-23), tomato mosaic virus resistance (Sm-4 and Sm-26) or fruit quality (Sm-4, Sm-26 and Sm-29), were crossed in all combinations (except selfings and Sm-4 × Sm-26) to produce five segregating hybrid populations. AFLP analysis of parental clones with four combinations of primers revealed 187 polymorphic bands out of 438 scored (42.7%). Genetic distance among parents varied between 0.325 (Sm-4 and Sm-23) and 0.210 (Sm-29 and Sm-26). For all traits studied (yield, fruit weight and soluble solids content), hybrid populations means ranged between those of the highest and lowest of the four parental clones. However, transgressive individuals for all traits could be found in almost all hybrid populations, and the mean of the 25% best indi...

Amplified Fragment Length Polymorphism (AFLP) analysis was used to generate informative DNA fingerprints from Magnolia denudata, M. liliiflora, and putative cultivars of their hybrid cross, i.e., M. × soulangeana, M. × soulangeana ’Lennei’, M. × soulangeana ’Rustica Rubra’, M. × soulangeana ’Alba’, M. × soulangeana Huby’, and M. × soulangeana ’San Jose’. A single specimen of unknown origin and representatives of the closely related M. campbellii and M. stellata were also included in the analysis. We show how this method may be used for distinguishing among cultivars.