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In order to combine the biological properties of oligonucleotides, a synthetic chemical modelized reaction was performed and the procedure then applied to the preparation of an alendronate-deoxyoligonucleotide conjugate through a carbamate linker.

Structural study using X-ray cristallography of the complexes of the title has been done. Alendronate is already known to be a very good chelator of divalent cations and supramolecular species obtained with alendronate and these cations are very stable and make them able to be used in biological systems. Our study points out that the size of the cation strongly modifies the structure of the complex. With Cu(II) and Zn(II) which are two cations with proximate ionic radii one cation is put in the centre of the coordination system and structures are isomorphous. On the other hand, with Cd(II), a binuclear complex is obtained. Results are discussed on the basis of these structures.

We describe a new preparation of tetraethyl but-3-enylidene-1,1-bisphosphonate 7. This preparation requires the phosphonylation of diethyl but-3-enyl-1-phosphonate which is obtained by the Michaelis-Becker reaction. Various methods of vic-diamination are studied to obtain tetraethyl 3,4-diaminobutylidene-1,1-bisphosphonate 11. The best approach consists in preparing the diazide by action of sodium azide, in the presence of manganese (III) acetate, and by reducing it in solution in tetrahydrofurane by triphenylphosphine in the presence of water. Hydrolysis by concentrated hydrochloric acid produces 3,4-diaminobutylidenel, 1-bisphosphonic acid 3.