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This study investigated the association between inflammation in infertile women and the risk of IVF-ET failure, as well as the potential effects of various lifestyles on this association. A total o...

The aim of this work was to study the expression of the cytokines IFN-γ, IL-4, IL-17 A, and TGF-β1 in peripheral blood and follicular fluid (FF) of patients positive for antithyroid autoantibodies (ATA摘要 这项工作的目的是研究甲状腺功能正常的抗甲状腺自身抗体阳性（ATA +）患者细胞因子IFN-γ, IL-4, IL-17A和TGF-b1在外周血和卵泡液（FF）中的表达, 以及探究这些自身抗体对体外受精和胚胎移植（IVF-ET）妊娠结局的影响。ATA +组中共计19名患者, 对照组为检测为抗甲状腺自身抗体（ATA-）阴性的27位患者。在取卵日, 移植后的第5天和第14天从两组患者中抽取血样, 另外, 在两组患者的取卵日提取卵泡液, 并通过酶联免疫吸附测定（ELISA）测量IFN-γ, IL-4, IL-17A和TGF-β1的表达水平。ATA +组在取卵日IFN-γ浓度增加, 外周血中TGF-β1浓度降低（p.05）；ATA +组移植后第5天和第14天外周血中IL-4浓度降低（p.05）; 在卵泡液中, IL-17A浓度增加而TGF-β1浓度降低（p.05）。 在取卵日和移植后第14天, ATA +组卵泡液和外周血中IL-17A / TGF-β1的比例显著增加（p.05）。 妊娠患者卵泡液IL-17 A / TGF-β1的比例显著低于非妊娠患者（p.05）。 研究结果表明, ATA +患者促炎细胞因子和抗炎细胞因子比例受到了不利影响; 接受IVF-ET治疗的ATA +患者的不良妊娠结局可归因于免疫机制。.

To identify the quantitative profiling of adiponectin and its receptors (AdipoR1, AdipoR2, and T-cadherin) in cumulus cells (CCs) and to evaluate their roles in the early embryo development of polycystic ovary syndrome (PCOS) patients, in part, with obesity.Fifty-five subjects were divided into two groups according to the body mass index. Oocytes were further inseminated and only mature and normal fertilized oocytes (2PN) were included in this research. Real-time PCR and western blot were performed to identify adiponectin and its receptors in CCs.Adiponectin and receptors were ubiquitously expressed in CCs of PCOS and non-PCOS patients. The level of AdipoR2 in CCs from the oocytes yielding blastocyst after 5/6 days in vitro culture was markedly higher than in those from oocytes could not develop to blastocyst stage after Day 6, for non-obese or obese PCOS patients (0.1647 ± 0.0161 versus 0.0783 ± 0.0385, 0.1948 ± 0.0307 versus 0.1057 ± 0.0236, respectively, p 0.05). In addition, only in patients with PCOS and concurrent obesity the AdipoR1 in CCs was considerably increased in CC-BThe development of early embryo was associated with the up-regulation of AdipoR1 and AdipoR2 in PCOS patients. Our results suggested that adiponectin could positively modulate embryo development in humans. Further investigations should be carried out to unlock the crucial role that adiponectin plays in embryo development.

γ-Aminobutyric acid (GABA) is a principal inhibitory neurotransmitter in the central nervous system (CNS) of vertebrates. However, GABA and its receptor are found not only in peripheral neuronal tissue but also in many peripheral nonneuronal tissues, and thought to have multiple physiological functions. The bidirectional communication between oocytes and cumulus cells (CCs) plays a significant role in oocyte maturation and metabolism. In our previously study, the expression level of α5 subunit in CCs isolated from oocytes of patients with polycystic ovary syndrome had been found to be associated with oocyte nuclear maturity. In this study, we investigated the transcriptional levels of GABAA receptor subunits in germinal vesicle (GV) and metaphase II (MII) mouse CCs, and explored the role of GABA-A receptor subunits during ovarian follicular development and oocyte maturation. We found that GABAA receptor subunits exhibited differential transcriptional levels in CCs at different oocyte nuclear maturity stages. It suggested an involvement of GABA-A receptor subunits related to oocyte maturation and certain functions.