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A new and simple HPLC-ELSD method for the determination of arbekacin sulfate injection and its related substances was developed. The column was Agilent SB-C18 (250 × 4.6 mm, 5μm). The mobile phase was 200 mM trifluoroacetic acid – acetonitrile (91:9) flow rate was 0.8 mL min−1. The detector used was an Dikma SEDEX 75 ELSD detector. The drift tube temperature was 50°C. The pressure of nebulizing gas was 3.5 bar. Good separation of arbekacin from the main related substances could be achieved. The standard curve was rectilinear in the range of 500 ∼ 2500 μg mL−1 (r = 0.9990). The average recovery of arbekacin is 100.8% (R.S.D = 1.5%, n = 9). The average recovery of dibekacin is 104.7% (R.S.D = 2.5%, n = 9). The limit of detection of arbekacin was 4.5 μg mL−1. The limit of detection of dibekacin was 5.0 μg mL−1. The method is simple and rapid, and the results are accurate and reproducible.

The vestibular toxicity of two aminoglycoside antibiotics, dibekacin sulfate and habekacin sulfate, and of a drug with potent antimitotic activity, cisplatin (cis-diamminedichloroplatinum) has been investigated in both rats and frogs. In rats, chronic intraperitoneal injection of a saline solution of dibekacin (50 mg/kg/day), habekacin (50 mg/kg/day), cisplatin (0.5 mg/kg/day) for 4 weeks and of cisplatin (1 mg/kg/day) for 5 weeks, produced no behavioral vestibular disorders and the righting reflex could be elicited at any time. In frogs, the spontaneous discharge was recorded from individual fibres of the ampullary nerve of the horizontal semicircular canal before and after acute administration of the drugs, dissolved in Ringer, into the perilymph of the inner ear near the horizontal ampulla. Following injection of 1 microliter of solutions containing 10 micrograms or 20 micrograms of dibekacin, 20 micrograms or 50 micrograms of habekacin, 0.5 micrograms, 2.5 micrograms or 10 micrograms of cisplatin, the spontaneous discharge decreased in a number of fibres and was sometimes completely abolished. The vestibular toxicity of the three drugs tested is discussed with respect to that of aminosides whose ototoxicity is well known.

The cochleo- and vestibulotoxicities of a new aminoglyco-sidic antibiotic, dibekacin, have been compared, in the guinea pig, with those of gentamicin and tobramycin. Besides an untreated control group, three groups of pigmented guinea pigs have been treated with 20 consecutive daily subcutaneous injections of one of these antibiotics respectively, with a dosage of 90 mg/kg/day (30 times the recommended human therapeutic dose which is the same for the three aminosides studied: 3 mg/kg/day). The auditory and vestibular functions in the awake animal were studied respectively by the measurement of thresholds of click-evoked responses at the auditory cortex and by the recording of eye movements (electronystagmography) during damped sinusoidal rotatory stimulation. These functions were monitored before, during and after the treatments in a few guinea pigs in each group, chronically implanted with auditory cortex and periocular electrodes. These functions were assessed only at the end of the experiments in the o...