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This paper describes the development and evaluation of an inhalable bioaerosol manifold sampler for the collection of replicate samples over sequential sampling periods. Wind tunnel experiments at wind velocitiesor = 0.6 m/sec (or = approximately 120 ft/min) and at a sampling velocity of 0.6 m/sec yielded sampling efficiencies within 10% of the American Conference of Governmental Industrial Hygienists' inhalable curve up to particle diameters of about 18 microns and efficiencies within 18% for diameters up to 26 microns. At wind velocitiesor = 0.6 m/sec, a sampling velocity of 0.8 m/sec yielded efficiencies that were within 15% of the inhalable curve up to diameters of about 22 microns and within 20% for diameters up to 25 microns. Microscopic counts of 15.6-micron and 25.6-micron monodisperse polystyrene beads aerosolized in the wind tunnel and collected with the manifold sampler yielded precise concentrations (pooled CV = 9.0 and 5.3%, respectively) averaging 115% and 92%, respectively, of measurements with the APS Model 3310 aerodynamic particle sizer. Field tests in a cotton dust environment demonstrated the ability to generate a time/concentration profile of fungal spores similar to that indicated for cotton dust levels determined with horizontal elutriators. Cotton dust was sampled efficiently while cotton fibers were excluded. Field tests on a dairy farm indicated no statistically significant bias (p = 0.72) between spore concentrations determined with the manifold sampler and concentrations determined through a central test port.