1. Tracing a fat or sweet lifestyle - New insights on catabolic paths of intracellular Salmonella
- Author
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Jost Enninga, Magdalena Gil, Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was funded by the ANR (LabEx IBEID, projectStopBugEntry and project AutoHostPath) and the EuropeanCommission (Consolidator Grant EndoSubvert)., ANR-15-CE15-0017,StopBugEntry,Identification des nouvelles molécules cellulaires cibles pour combattre les infections bactériennes(2015), ANR-15-CE15-0018,AutoHostPath,Rôles alternatifs pour les récepteurs de l'autophagie dans les interactions hôte-pathogène(2015), European Project: 682809,EndoSubvert(2017), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Salmonella typhimurium ,0301 basic medicine ,Microbiology (medical) ,Cytoplasm ,Salmonella ,Immunology ,Biology ,medicine.disease_cause ,Microbiology ,Mice ,03 medical and health sciences ,Bacterial Proteins ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,medicine ,Animals ,Humans ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Promoter Regions, Genetic ,Life Style ,ComputingMilieux_MISCELLANEOUS ,2. Zero hunger ,Virulence ,Catabolism ,Life style ,Macrophages ,Gene Expression Regulation, Bacterial ,Flow Cytometry ,Adaptation, Physiological ,Editorial ,030104 developmental biology ,Infectious Diseases ,Biochemistry ,Vacuoles ,Parasitology ,Metabolic Networks and Pathways ,Intracellular ,Research Paper - Abstract
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative pathogen that causes various host-specific diseases. During their life cycle, Salmonellae survive frequent exposures to a variety of environmental stresses, e.g. carbon-source starvation. The virulence of this pathogen relies on its ability to establish a replicative niche, named Salmonella-containing vacuole, inside host cells. However, the microenvironment of the SCV and the bacterial metabolic pathways required during infection are largely undefined. In this work we developed different biological probes whose expression is modulated by the environment and the physiological state of the bacterium. We constructed transcriptional reporters by fusing promoter regions to the gfpmut3a gene to monitor the expression profile of genes involved in glucose utilization and lipid catabolism. The induction of these probes by a specific metabolic change was first tested in vitro, and then during different conditions of infection in macrophages. We were able to determine that Entner-Doudoroff is the main metabolic pathway utilized by Salmonella during infection in mouse macrophages. Furthermore, we found sub-populations of bacteria expressing genes involved in pathways for the utilization of different sources of carbon. These populations are modified in presence of different metabolizable substrates, suggesting the coexistence of Salmonella with diverse metabolic states during the infection.
- Published
- 2017
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