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Platelet-rich plasma (PRP) is a therapeutic option in different fields based on its growth factors. We investigated influence of PRP on islet survival, function, transplantation outcomes, and pancreatic genes expression in diabetic rats. In vitro: pancreatic isolated islets were incubated with/without PRP then viability, insulin secretion, and content were assessed. In vivo: Series 1 were designed to determine whether islet treatment with PRP improves transplantation outcome in diabetic rats by evaluating plasma glucose and insulin concentrations and oxidative parameters. Series 2, effects of PRP subcutaneous injection were evaluated on pancreatic genes expression and glucose tolerance test in diabetic rats. PRP enhanced viability and secretary function of islet. Reduced glucose and malondialdehyde levels as well as increased insulin levels, superoxide dismutase activity, and expressions of pdx1 and insulin were observed in diabetic rats. PRP treatment has positive effects on islet viability, function, transplantation outcome, and pancreatic genes expression in diabetic rats.

Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in infertile rat model by stereological method. Thirty-two male rats were divided into four groups. Infertility was induced by the administration of busulfan (BUS) (10mg/kg, I.P., single dose). PRP (80μl, testis local injected, single dose) was administered for the subjects. After 48 days, semen analysis was performed and blood samples were taken from the heart to measure the testosterone. Then, the left testis was removed, fixed, embedded, sectioned, and stained by HE; after that the testes were analyzed. The results showed that BUS can decrease the sperm count, motility, normal morphology, length of the spermatozoon tail, volume of the testis, seminiferous tubules, germinal epithelium height, and the number of spermatogenesis lineage cells in comparison with the control group (p 0.01). PRP increased the number of spermatogenic stem cell, count, motility and tail length of the sperm and testosterone level in BUS-treated animal significantly, but it did not have any effect on the volume of the testis, germinal epithelium height, Sertoli and Leydig cells number, and seminiferous tubules length. It concluded that PRP can improve the structural and functional impairment of the testis in treatment by BUS.

Numerous bioactive growth factors and cytokines in platelet-rich plasma (PRP) have recently made it an attractive biomaterial for therapeutic purposes. These growth factors have the potential to regenerate the injured tissues. The aim of this study was to investigate the therapeutic effects of PRP in hepatotoxic animal model. Hepatotoxicity was induced in rats by oral administration of 4 mL/kg/week of CCl4 diluted 1:1 in corn oil for 10 weeks. To confirm the hepatotoxicity, 24 h after the last CCl4 administration, blood samples were collected via cardiac puncture to assess the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein, and total bilirubin. Twenty-four hours after blood collection, the experimental animals received a single injection of PRP (1 mL) via the anterior mesenteric vein. One week later, all biochemical tests were performed again, and the rats were scarified and their livers were removed, prepared histologically, and stained. The stereological analyses were performed to evaluate the effects of PRP on histopathological features of CCl4-treated livers. The results were compared statistically with the corresponding control and CCl4+normal saline (NS)-treated animals. A significant decrease in the number and volume of hepatocytes (p = 0.01), and also a reduction in the volume of sinusoids (p = 0.001) and connective tissue (p = 0.04), were observed in the PRP-treated animals compared with the CCl4+NS-treated ones. Our findings demonstrated that application of PRP had beneficial effects on CCl4-induced fibrosis; however, it had detrimental effects on the total number of hepatocytes and the volume of hepatocytes and sinusoidal spaces.

To determine the effects of noise exposure on hearts of newborn mice, 48 pregnant mice were exposed to recorded aircraft flight noise (100 dB) for 150 min from the 2nd day of pregnancy to 7th day after delivery. Mice of the same age were selected as control group. The weights and crown-rump lengths of the fetuses and the newborns were measured, and their hearts were removed under deep anesthesia on the 1st, 7th, and 14th days after birth and prepared histologically. Quantitative changes of the hearts were measured stereologically by the Cavalieri method. The ultrastructural changes of cardiac myocytes were examined using transmission electron microscopy. No histological changes were observed, but the volumes of newborns’ hearts in the experimental groups increased significantly compared to the control group. Photomicrographs of cardiac muscle cells revealed nuclear envelope changes, chromatin condensation, and increased mitochondria and myofibrils in noise-exposed newborns. It is concluded that the newbor...

Talaei-Khozani, T., Monsefi, M., Vojdani, Z. and Dehghani, F. 2007. Histochemical study of the effects of noise on the cell surface and extracellular matrix glycoconjugates of the developing mouse cochlea. J. Appl. Anim. Res., 31: 209–212. To determine whether noise could change the glycoconjugate pattern of developing cochlea 42 pregnant mice (2–4 m) were exposed to 100 dB continual sound for 2.5 h daily. After parturition, the cochlea of the offspring in the first, seventh and fourteenth day after birth were removed, prepared histologically and stained with lectins, WGA (wheat germ agglutinin), PNA (peanut agglutinin), GSA (Griffonia simplicifolia) and DBA (Dolichos biflorus agglutinin). Exposure to noise led to decrease in the uptake of the PNA by spiral limbus and ligament in the seventh and fourteenth postnatal days. It is concluded that alternation in glycosilation of glycoconjugates in the noise exposed cochlea could be responsible for the structural and functional changes such as fetal no...