1. Involvement of tumor-associated macrophage activationin vitroduring development of a novel mantle cell lymphoma cell line, PF-1, derived from a typical patient with relapsed disease
- Author
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Alex Rollo, Muychi T Vang, Michael Wang, Archito T. Tamayo, Liang Zhang, Zhishuo Ou, Richard J. Ford, Jeffrey L. Jorgensen, Lan V. Pham, Gary Lu, and Pramoda Challagundla
- Subjects
Cancer Research ,Tumor cells ,Lymphoma, Mantle-Cell ,Tumor-associated macrophage ,Biology ,Article ,Immunophenotyping ,Pathogenesis ,immune system diseases ,In vivo ,Cell Line, Tumor ,hemic and lymphatic diseases ,medicine ,Humans ,neoplasms ,In Situ Hybridization, Fluorescence ,Chromosome Aberrations ,Macrophages ,Hematology ,RELAPSED DISEASE ,Macrophage Activation ,medicine.disease ,In vitro ,Cell biology ,Oncology ,Cell culture ,Antigens, Surface ,Cancer research ,Mantle cell lymphoma ,Neoplasm Recurrence, Local - Abstract
Human mantle cell lymphoma (MCL) cell lines are scarce and have been only sporadically described and validated, and only a few have been thoroughly molecularly or genetically characterized. We describe here the successful establishment of a new MCL line, PF-1, with typical MCL characteristics. Culturing primary MCL cells in vitro initially gave rise to an essential generative microenvironment “niche” involving macrophages required for MCL growth, and eventually produced the PF-1 MCL cell line. Our analysis revealed that PF-1 is morphologically and genotypically nearly identical to the original tumor cells. The PF-1 MCL cell line that we have developed will be useful for in vitro and in vivo studies of MCL pathogenesis and therapeutics.
- Published
- 2014