1. From sample to eluate; a validation study of Qiagen® QIAsymphony® DNA Investigator® Kit, a magnetic bead chemistry, on Hamilton® Microlab STAR® Autolys®
- Author
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Marco Pizzamiglio, Giampietro Lago, Stella Eugenia Cirillo, Richard Kessell, Massimiliano Stabile, and Christian Faccinetto
- Subjects
Engineering ,Chromatography ,Lysis ,Elution ,business.industry ,Sample (material) ,Extraction (chemistry) ,DNA extraction ,Pathology and Forensic Medicine ,chemistry.chemical_compound ,chemistry ,Magnetic bead ,Forensic profiling ,Forensic engineering ,business ,DNA - Abstract
DNA extraction is arguably the most critical stage in the process of forensic profiling. If done poorly, the sample can be lost and all downstream processing steps will be fruitless as they are dependent on the quality of the extract. The main factors to take into consideration to ensure a good quality of extracted DNA are: the efficiency of the extraction method and the absence of cross-contamination between samples. In this article we will discuss the implementation and validation of a fully automated DNA extraction method that involves the use of Qiagen® QIAsymphony® DNA Investigator® kit being processed on a Hamilton® Microlab STAR® automated platform in conjunction with Autolys® technology (barcoded Autolys® tubes used for the initial lysis, and subsequent filtration of lysates). Forensic trace samples are first placed on the Hamilton® Microlab STAR® Autolys® in Autolys® tubes by the operator. After the lysis and extraction process, the DNA extracts are collected into tubes with unique two-di...
- Published
- 2015