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This study was carried out to evaluate the effect of extraction methods (direct maceration (DM) and successive maceration (SM)) and solvents (dichloromethane (DCM), ethyl acetate (EAc), butanol (Bu...

The essential oils extracted by Clavenger apparatus from leaves of Rosmarinus officinalis cultivated in different areas in Djerba (Island in the southern of Tunisia) were evaluated for their chemical composition (GC and GC-MS methods), antioxidant (DPPH method) and antibacterial activities (agar-well diffusion method). GC-MS analysis revealed the presence of three chemotypes of oils: 1,8-cineole, 1,8-cineole/camphor/α-pinene/camphene and 1,8-cineole/camphor/α-pinene/ verbenone/borneol which was not previously detected in Tunisian Rosmarinus officinalis. The three chemtypes exhibited moderate antioxidant activity with an IC50 ranged from 4.186 mg/ml for chemotype I to 7.298 for chemotype II and showed strong to moderate antibacterial activity against the six bacterial strains tested with a MIC ranged from 0.156 to 1.25 mg/mL (Chemotype I and chemotype II) and from 1.25 to 5 mg/mL (chemotype III). This study showed that the essential oil extracted from Rosmarinus officinalis cultivated in Djerba ha...

Seedlings of Artemisia herba-alba Asso collected from Kirchaou area were transplanted in an experimental garden near the Institut des Regions Arides of Medenine (Tunisia). During three years, the aerial parts were harvested (three levels of cutting, 25%, 50%, and 75% of the plant), at full blossom and during the vegetative stage. The essential oil was isolated by hydrodistillation and its chemical composition was determined by GC (RI) and 13C-NMR. With respect to the quantity of vegetable material and the yield of hydrodistillation, it appears that the best results were obtained for plants cut at 50% of their height and during the full blossom. The chemical composition of the essential oil was dominated by b-thujone, a-thujone, 1,8-cineole, camphor and trans-sabinyl acetate, irrespective of the level of cutting and the period of harvest. It remains similar to htat of plants growing wild in the same area.