1. Isolation and characterization of the single β-tubulin gene inPhytophthora cinnamomi
- Author
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N. D. Weerakoon, J. S. Marshall, J. M. Wilkinson, Adrienne R. Hardham, L. P. Lehnen, and J. K. Roberts
- Subjects
0106 biological sciences ,0301 basic medicine ,Physiology ,macromolecular substances ,Phytophthora cinnamomi ,010603 evolutionary biology ,01 natural sciences ,Genome ,Microbiology ,03 medical and health sciences ,Phylogenetics ,Genetics ,Molecular Biology ,Gene ,Ecology, Evolution, Behavior and Systematics ,Southern blot ,Oomycete ,biology ,fungi ,Intron ,Cell Biology ,General Medicine ,030108 mycology & parasitology ,biology.organism_classification ,Phytophthora - Abstract
To initiate studies on the regulation of tu- bulin expression and microtubule organization in the phytopathogenic Oomycete, Phytophthora cinna- momi, a gene encoding 3-tubulin was cloned and se- quenced. The gene has no introns and encodes a protein of 445 amino acids. Genomic Southern blots indicate a single copy of the 3-tubulin gene in the P cinnamomi genome. Comparison of the P cinnamomi B3-tubulin gene sequence with B3-tubulin genes from a range of fungi, algae and protists supports the hy- pothesis that the Oomycetes are more closely related to the algae and protists than they are to the higher fungi. more...
- Published
- 1998
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