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A HPLC method for determination of the bitter principles, bitrex, quassia and sucrose octaacetate, next to other ingredients in Specially Denatured Alcohol formulations is described. The method is based on evaporation of a sample, extraction of the residue with hexane and analysis of the extracted residue on a Cyano-type column with acetonitrile-water used as the eluent. Baseline separation of compounds and satisfactory quantitation has been achieved. Samples without pretreatment can occasionally be analysed with slight modifications of the procedure.

Evaluation of Calibration Table and Calibration Averaging Routines developed by Waters Associates for quantitative determination of sucrose, glucose and fructose using Waters Associates Sugar Analyzer is discussed. Both routines are sufficiently accurate and offer some advantages over previously-described Lane-Eynon Method of determination of reducing sugars by means of Fehling Solution with Methylene Blue as internal indicator. However, great care must be taken to prevent sucrose inversion on Sugar-PAK I column, which could otherwise render the quantitation of sugars useless.