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The importance of biofuels is growing for both environmental and economic point of view. The usage of wastes in bio-ethanol production is investigated in our paper. Cellulose contained wastes i.e. byproduct of tobacco is used for experiments. The paper is focused mainly on the investigation of the enzyme recovery that followed the saccharification process. The membrane filtration combined with sonication was used for enzyme recovery. Our results show that recovered enzyme did not lose its activity. The patterns were originated from fermented solutions made from tobacco byproducts. Based on our results, the ultrasound accompanied by a membrane separation would be a possible and effective operation of bio-ethanol production technology.

The development of second-generation biofuels from cellulosic/lignocellulosic biomass has advantages from energy and environmental aspects, but the overall cost of the process is mainly dependent on the cost of the enzymes. Enzyme recovery and recycling is one of the most important and effective means of increasing the efficiency of enzymatic hydrolysis processes by lowering the enzyme costs. The primary objective of this study was to investigate the possibilities of enzyme recovery by membrane separation. Ultrafiltration (UF) membranes with various cut-off values and materials were used to recycle cellulase and cellobiase in model solutions and cellulosic hydrolysates. The membrane separation process was followed by determination of the flux, and its efficiency of it was measured via sugar and protein retention, and the resistances were also calculated. A polyether–sulfone membrane with a cut-off value of 5 kDa, (PES5) operated at 26.8 L m−2 h−1 with 87.3% protein rejection while a thin-film mem...