1. Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing
- Author
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Ni,Qin, Yao,Xingwei, Li,Jinhui, Ma,Jinghan, Wang,Kaiying, Liu,Xiong, Li,Peihan, Yang,Lang, Li,Peng, and Li,Shenlong
- Subjects
Pharmacology ,Infectious Diseases ,Infection and Drug Resistance ,Pharmacology (medical) - Abstract
Qin Ni,1,2,* Xingwei Yao,3,* Jinhui Li,2,* Jinghan Ma,3,* Kaiying Wang,2 Xiong Liu,2 Peihan Li,2 Lang Yang,2 Peng Li,2 Shenlong Li1,2 1Epidemiology, Zhengzhou University, Zhengzhou, Peopleâs Republic of China; 2Biosafety, Chinese PLA Center for Disease Control and Prevention, Beijing, Peopleâs Republic of China; 3Medical Clinical Laboratory, Dong Zhimen Hospital Beijing University of Chinese Medicine, Beijing, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Peng Li; Shenlong Li, Email jiekenlee@126.com; lishenlong@sohu.comObjective: Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing.Methods: Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes.Results: All strains were multi-drug resistant. Twenty-two strains carried the blaKPC-2 gene and two harbored blaNDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII (pHN7A8), IncX, or IncFIB (K).Conclusion: This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens.Keywords: Klebsiella pneumoniae, drug resistance, carbapenemase, ST11, blaKPC-2
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- 2022