1. Chemoprevention assessment, genotoxicity and cytotoxicity of flavonoids from Inga laurina leaves (FABACEAE)
- Author
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T O R Sanzovo, Rodolfo Bortolozo Serafim, Nerilson M. Lima, Juliana Maria Sorbo, Christiane Pienna Soares, S. R. Marqui, Valeria Valente, Geovana Navegante, Dulce Helena Siqueira Silva, Teresinha de Jesus Aguiar dos Santos Andrade, Universidade Estadual Paulista (Unesp), and Science and Technology of Maranhão
- Subjects
Plant Science ,Reductase ,Pharmacology ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,medicine ,chemoprevention ,Cytotoxicity ,Flavonoids ,chemistry.chemical_classification ,010405 organic chemistry ,genotoxicity ,Organic Chemistry ,food and beverages ,0104 chemical sciences ,Quinone ,Comet assay ,010404 medicinal & biomolecular chemistry ,Enzyme ,chemistry ,Chemoprotective ,cytotoxicity ,Inga laurina ,Genotoxicity - Abstract
Made available in DSpace on 2020-12-12T01:45:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 This study aimed to identify and evaluate the cytotoxicity, genotoxicity, antigenotoxicity and chemoprevention assessment of flavonoids myricetin-3-O-(2″-O-galloyl)-α-rhamnopyranoside and myricetin-3-rhamnoside from Inga laurina leaves extract. The Quinone reductase induction as a biomarker for cancer chemoprevention was evaluated in murine hepatocellular carcinoma, the cytotoxicity was evaluated by sulforhonamide B assay and genotoxicity was evaluated by comet assay using HepG2 cell line. The results demonstrated that the flavonoids didn´t show cytotoxicity in HepG2 cells. In the chemoprevention evaluations were not able to promote the induction of Quinone Reductase and also no genotoxic effect was observed by evaluation of the comet assay in none of the concentrations tested. In the antigenotoxicity test, all compounds had a protective effect against damage induced by hydrogen peroxide and were repaired against damage. Although none of the flavonoids were capable of inducing the enzyme Quinone Reductase at the concentrations tested, the antigenotoxicity results showed a powerful chemoprotective action. School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University UNESP Institute of Chemistry Nucleus Bioassays Biosynthesis & Ecophysiology of Natural Products São Paulo State University UNESP NIAC-Nucleus of Research Applied to Sciences-IFMA-Federal Institute of Education Science and Technology of Maranhão School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University UNESP Institute of Chemistry Nucleus Bioassays Biosynthesis & Ecophysiology of Natural Products São Paulo State University UNESP
- Published
- 2019
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