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Brown foundation seaweeds are key elements increasing substrate heterogeneity and shaping the biodiversity in rocky coastal ecosystems. They are, however, vulnerable species that are declining due to multiple anthropogenic and climate change stressors, leading to a shift to less structural complex habitats. We investigate the role of structural attributes of two intertidal macroalgae, Ericaria amentacea and Laurencia obtusa, in shaping the abundance and diversity of their associated epifaunal assemblages. For this aim, we measured seaweeds’ biomass, thallus volume and length (used here as proxy of substrate complexity), and explored which seaweeds’ substrate attribute explained better variation of epifaunal assemblages. Results showed that E. amentacea was more complex than L. obtusa and hosted a higher number of epifaunal individuals. However, unlike that expected, the epifaunal assemblage of L. obtusa was more structured with higher Shannon–Wiener diversity and Pielous’ evenness. Our findings indicate that, besides seaweed’s substrate attributes, other mechanisms such as wave action and chemical defense might play a role in structuring epifaunal assemblages. We suggest that a shift from E. amentacea to L. obtusa population could have effect on structure and abundance of associated epifaunal assemblages. Certainly, further investigations are needed to clear up the consequences of these changes.

Although Astragalus nebrodensis plays a fundamental ecological role, mycorrhizal fungi associated with this species have never been investigated in natural habitats. Our aim was to check the presence and occurrence of Arbuscular Mycorrhizal Fungi (AMF) in roots of A. nebrodensis in a natural habitat and to screen the fungal diversity in the surrounding soil, considering the positive influence of mycotrophic shrub species on soil microbiota. A morphological analysis was performed on A. nebrodensis roots samples from the Madonie mountains (Sicily, Italy), while high-throughput-sequencing was carried out in A. nebrodensis roots and in the associated soil. Observations of A. nebrodensis roots revealed typical structures of AMF. Sequencing revealed that Ascomycota was the most abundant phylum in both roots and soil samples, followed by Basidiomycota and Mucoromycota. A. nebrodensis roots host a fungal community with lower richness as compared to soil and specific taxa were differentially abundant between roots and soil. The endomycorrhizal symbiosis in A. nebrodensis from a natural habitat is reported here for the first time. The fungal diversity between the two matrices (soil vs roots) supports the notion of a specialised and well-established root microbiome in A. nebrodensis enabling it to survive the stressful conditions of its harsh environment.

Helianthemum lippii is a perennial shrubby plant growing in the sandy environments of Italy, Mediterranean countries of North Africa and Middle East. H. lippii is used in traditional medicine but there are very few reports referring to the phytochemical characterization, the ethnopharmacology, and the biological activity of H. lippii. The goal of this study was todetermine the phytochemical composition of different H. lippii extracts, cold (CME) and hot (HME) methanol, cold (CPEE) and hot (HPEE) petroleum ether, as well as to evaluate their anticancer and antimicrobial activities and biofilm formation reduction. Fifty-fours phytocompounds have been determined by HPLC-UV-ESI-QTOF-MS analysis. All the four extracts reduced the viability of human MDA-MB231 and HCT116 cells, being cold and hot methanol extracts the most effective. The antimicrobial activity against S. aureus, P. aeruginosa, E. faecalis, E. coli, C. albicans was also evaluated. Data showed the greater susceptibility of S. aureus to hot methanolic extract. Concerning antifungal activity, C. albicans resulted more susceptible to petroleum ether. Moreover, some of the samples exhibited a good antibiofilm activity both on immature and on mature biofilms. The four extracts showed interesting antimicrobial and cytotoxic activities and can be considered good candidates for new therapeutic applications

The results of the 4th National Report for the Italian flora under the 92/43/EEC ‘Habitats’ Directive are presented. The outcomes showed a general negative conservation status for plant species, with the worst situation being in the Mediterranean bioregion. At the National level, significant monitoring and conservation activities are required.

The antibacterial activity of methanolic extracts of eight Mediterranean brown algae belonging to the genera Cystoseira sensu lato, Dictyopteris and Padina was investigated. Extracts from Sargassaceae showed antibacterial activity. Specifically, Carpodesmia crinita and Carpodesmia brachycarpa extracts inhibited the growth of the Gram-positive strain Kocuria rhizophila whereas Cystoseira compressa and Carpodesmia amentacea extracts showed antibacterial activity against the Gram-positive strains, Kocuria rhizophila and Staphylococcus aureus. None of them inhibited the growth of the Gram-negative bacterium Escherichia coli. The extracts of Cystoseira sp. pl. and Treptacantha ballesterosii did not show any antibacterial activity. HPLC-MS analysis of the extracts allowed identification of polar compounds. Taxa of the genus Cystoseira sensu lato showed a higher number of compounds than Dictyopteris poly- podioides and Padina pavonica. Some differences found among and within taxa can be attributed to the different depths where these algae live, to thallus morphology and herbivore pressure. Particularly interesting were the phenol compounds, which showed differences among the genera but also within the genus Cystoseira sensu lato. Noteworthy, the almost exclusive presence of the fukiik acid isomer and of bromo-phloroglucinol in Carpodesmia amentacea, and the presence of exifone, able to bind iron metal ions, in Carpodesmia amentacea, Cystoseira compressa and Treptacantha ballesterosii.

This article presents a comprehensive inventory of the urban vascular flora of the town of Palermo. The data were gathered from extensive field collections, from specimens kept in different herbaria, and publications of the last 30 years. The floristic catalogue includes all the vascular plants that occur spontaneously within the urban area. Thirteen taxa are endemic to Sicily and one of these, Clinopodium raimondoi, is exclusive to Palermo’s area. The inventory comprises 1052 taxa belonging to 119 families and 225 genera, and contains 170 taxa non-native to the Italian flora.

The rare fungus Hericium erinaceus (Bull.) Pers. was collected from temperate forests in northwestern Tunisia and described for the first time in Africa. In this paper, we report data about the distribution, ecology, morphology and molecular identification of H. erinaceus. Collected data may help expand our knowledge on this critically endangered rare species worldwide.

The chief purpose of Marine Protected Areas (MPAs) is biodiversity conservation. The effects that invasive alien species (IAS) have on MPAs, and vice versa, are not yet fully known, even though assessing them is crucial for MPA planning. Management plans require sound knowledge of the pathways of introduction, the impact and current distribution of IAS. Monitoring plans are essential for preventing and reducing the risk of IAS introduction. In this respect, the involvement of citizen scientists in gathering data (validated by taxonomic experts) on the occurrence of IAS, that would otherwise be impossible to collect, may be crucial. We report on our experience of citizen science in the Egadi Islands MPA with the project ‘Caulerpa cylindracea – Egadi Islands’, aimed at monitoring the spread dynamics of the ‘sea grape’ C. cylindracea. The project registered 156 sightings and also allowed collection of records and information concerning other non-indigenous and cryptogenic species, e.g. the spotted sea hare (Aplysia dactylomela), the harpoon weed (Asparagopsis armata), the red sea plume (Asparagopsis taxiformis), the tube-building sabellid (Branchiomma bairdi), the blue spotted cornet fsh (Fistularia commersoni) and the nomad jellyfsh (Rhopilema nomadica).

The conservation, fruition and management of landscape and natural resources is one of the most important topic of the moment. Here we presented a study on naturalistic hotspots that can be found along the Itinerarium Rosaliae, a naturalistic and religious route in Sicily that crosses three natural reserves and one natural regional park connecting Mount Pellegrino with the Hermitage of Santo Stefano di Quisquina. On the whole 7 semi-natural woods, 13 monumental trees, 8 geosites, and 4 areas of high naturalistic interest were identified. This initiative can be of example for other territories where human and natural heritage can be connected for an organic network of fruition.

New data on the geographical distribution and ecology of two rare taxa of Carex collected in the area of the Nebrodi Mountains (Sicily) are provided; the Sicilian populations of both taxa are at the border of their respective ranges, and their regional distribution is still poorly known. The new population of Carex x boenninghausiana reported here confirms the presence of this hybrid in Sicily, but at the same time it represents the only known population currently occurring in Italy; in fact, the other - so far only - known Italian population is considered extinct. The second report concerns Carex grioletii, recently considered doubtful for the Sicilian flora, whose new population here reported is the only confirmed and currently present population in the island; the assessment of extinction risk and conservation status, carried out at regional (Sicilian) level according to the IUCN criteria for the latter taxon, showed that it is Critically Endangered (CR).

Tamarix macrocarpa, a species with distribution in Central and North Africa, in the countries bordering the Persian Gulf and in Central and western Asia is recorded for the first time for Europe. Pentamerous flowers and androecium diplostemonous characterize this small tree.

We used 39 morphological and anatomical needle traits in the biometric comparisons Abies nebrodensis with A. alba and A. cephalonica. The multivariate analyses were utilised and a closer relationship of A. nebrodensis to A. cephalonica than to A. alba was detected, in contrast to what has been shown for cone characteristics.

So far considered as endemic to Greece, Hieracium umbrosum subsp. abietinum is reported as new for the Italian flora. Its presence has been noted on the Pollino National Park (S Italy). Morphological, ecological and taxonomical notes are given. This discovery increases the list of taxa showing an amphi-Adriatic distribution.

Pancratium maritimum L. (Amaryllidaceae) is a geophyte occurring in the Mediterranean region, from the Black Sea to part of the Atlantic coast. This plant is receiving much attention from the international scientific community due to its value as a bioindicator, the potential industrial value of its chemical compounds, and its use as a commercial ornamental plant. Plant morphometry and sequences of three plastid DNA regions (rbcL, matK, trnH-psbA) were used to assess the phenotypic and genetic variability of this taxon and its closest congeneric species (in particular Pancratium linosae, from the volcanic island of Linosa) in the Central Mediterranean (Sicily, Tunisia and surrounding islands). Pancratium maritimum and P. linosae cannot be distinguished based on morphological and genetic data and should belong to the same taxon. Our results also highlight a diversified gene pool in P. maritimum that is worth preserving. The lectotypes of the names Halmira stellaris, Pancratium angustifolium and Pancratium foetidum are here designated.

An update on the taxonomy and geographic distribution of the genus Thymus in Sicily is given. This study is based on literature, herbarium and field investigations. In total, five taxa belonging to Thymus sect. Serpyllum (Th. richardii subsp. nitidus, Th. longicaulis and Th. praecox subsp. parvulus) and Th. sect. Hyphodromi (Th. spinulosus and Th. paronychioides) occur in Sicily. For each one, information about taxonomy, habitat, phenology, chromosome number and distribution are provided. Furthermore, the name Thymus pedicillatus Lojac. is here typified. Finally, an analytical key for the identification of the studied taxa is proposed.

Ruppia cirrhosa growth cycle was analysed in a southern Mediterranean shallow system throughout 1 year. We examined the temporal variation in R. cirrhosa cover percentage, shoot density, biomass, leaf length, no. flowers m-2 and no. fruits m-2 in two groups of pond characterized by differences in some environmental parameters. Ponds were comparable for salinity and temperature but they differed for other environmental parameters such as water depth, level of suspended organic matter and chlorophyll a (CHL a). Biological parameter values were higher in B ponds, characterized by lower values of water depth, suspended organic matter and CHL a. A seasonal trend for all considered biological parameters in both typologies of ponds with maximum values in summer was also observed. Moreover, differences were observed between the two groups of ponds in relation to the reproductive strategy adopted by the plant, with populations subjected to a higher organic input and a lower water depth displaying an annual cycle. Results showed how R. cirrhosa is able to resist and to adapt to variations in environmental conditions because of the plasticity and flexibility in the growth cycle and in the reproductive effort.

Seed lipids composition is a tool to discriminate among plant taxa and is related to phylogeny and biogeographic distribution. Aim of the present study was to evaluate the fatty acid (FA) composition of the seed lipids from nine natural Chamaerops humilis L. population and its relation with the climatic traits of the collection sites. The average seed lipids content was 54.8 g kg21 and the most represented FAs were oleic (478 g kg21 oil) and linoleic (230 g kg21 oil), with significant differences among the accessions. Most of the lipid traits significantly correlated with climatic traits. In particular, oleic acid negatively correlated with year potential evapotranspiration. These relations could emerge from a genotypic adaptation to the environment. The seed of C. humilis showed high content of lipids, which implies an importance of the species for feeding wild life during winter, and a high degree of unsaturation. Considering the importance of the unsaturated FAs in human and animal nutrition, the present results suggest that European fan palm could be introduced in breeding programmes and its seed extract used as FA in diet supplementation. Further studies are needed to identify the content of anti-nutritional or nutraceutical compounds.

The work on the Isoetaceae account for the Critical Flora of Italy has resulted in a new, synonymic inventory of Italian Isoetes taxa, based on herbarium studies, observation by scanning electron microscopy and critical re-examination of the previously published works. Ten species are listed. The names I. longissima and I. gymnocarpa are restored as correct, to replace I. velata and I. subinermis, respectively. Eighteen lectotypes, one epitype and one neotype are designated. A dichotomous key to Italian taxa is provided.

Names of 20 presumed taxa in Draba sect. Aizopsis, all based on material from Italy, are considered. Full synonymies are provided, and types are designated (for 14 names) or indicated. Most of the taxa are currently considered unworthy of recognition, of which 16 belong to D. aspera sensu lato. However, the question of whether the Sicilian populations might be distinct from the peninsular populations is still unsettled.

We characterized a new Gracilaria species from the Venice Lagoon, Italy, using molecular analyses based on the plastid large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase gene (rbcL) and the intergenic RuBisCO spacer (rbcL-rbcS), combined with morphology data. This new entity was recorded on the artificial substrata of the Venice Gulf from March to July, adding to 12 Gracilaria taxa already recorded in the Mediterranean and Adriatic seas. Thalli exhibited a green-yellowish pigmentation with pink shades, and there was dense branching in the distal portions. Tetrasporangia were scattered on thallus cortex and distributed mostly on short, stipitate branchlets. The inner pericarp was connected to the gonimoblast by tubular nutritive cells. The male gametophytic plants formed roundelliptical spermatangial verrucosa-type conceptacles. This species grew attached on artificial rocky substrata of the low midlittoral and upper sublittoral zone in spring and early summer. Molecular analyses based on the plastid-encoded rbcL gene showed a 99.66% nucleotide identity with another Gracilaria sp. from southern Sicily. We compared our rbcL-rbcS spacer sequences with those of two cryptic species, and the phylogenetic analyses confirmed that the Venice populations were a new species. We suggested that the discovery of this new species was not due to an extraMediterranean introduction but the consequence of its misidentification as Gracilaria gracilis, which has a similar gross morphology.

Five populations belonging to three subspecies of Dianthus rupicola Biv. (D. rupicola subsp. rupicola, D. rupicola subsp. aeolicus, and D. rupicola subsp. lopadusanus) and growing in different geographical areas of Sicily were tested for seed germination at various temperatures. All populations showed high germination rates with an optimum temperature between 15°C and 25°C. Efficient seed germination might contribute significantly to the preservation of these subspecies which are currently exposed to several environmental threats.

In this study, Sorbus busambarensis, a new species from Rocca Busambra (Palermo, NW Sicily) has been described and named. On the basis of the critical features of its flowers, fruits and leaves, it is noted to be a member of S. sect. Aria and is related to the other taxa that form the polymorphic group of S. aria, which in Sicily is represented by S. aria s. str., S. graeca and S. umbellata.

Current knowledge about Italian wood-decay fungi (Basidiomycota and Ascomycota) is surveyed: 1582 taxa belonging to Ascomycota (341) and Basidiomycota (1241) have been reported, including 23 species new to science of Basidiomycota described from Italy within the last five years. Evaluating diversity of wood-decay fungi can provide a more accurate estimation of species richness for fungi which are an important functional component of ecosystems. Aphyllophoroid and Ascomycota species play an important role in habitat conservation and management. Sardinia, Sicily, the Alps and the Apennines are “hot spots” for wood-decay Basidiomycota in Italy.

The concentration of rare-earth elements and yttrium (REY) was investigated in dissolved phase, suspended particulate matter, and seafloor sediments of the western coastal area of the Gulf of Thailand. The samples show Eu and Gd positive anomalies in the shale-normalized REY patterns, especially in the suspended particulate matter. On the other hand, a very high REE content was detected in the coastal waters, probably due to the weathering produced by the Mae Klong river waters on rare-earth element (REE)-rich accessory minerals coming from terrains and mineral deposits cropping out in the studied area. The shale-normalized patterns of yttrium and REE estimated for the dissolved phase show an enrichment of medium rare-earth elements (MREE), characteristic of extensive water–rock interactions and weathering occurring in the continental environment. The REE concentrations of suspended particulate, normalized to the REE concentrations in each sediment sampled from the tidal flat, show the same behaviour of the experimentally determined apparent REE bulk distribution coefficients 1 for pH values ranging from about 5.5 to 6.2. Since the REY concentration of the water masses is controlled by the oceanographic features of the studied area, riverine inputs, and ionic straight, we suppose that the dissolved phase represents a mix of truly dissolved and colloidal pool (

Sect. Capparis is represented by a single species, Capparis spinosa L., divided into several intraspecific taxa showing plesiomorphic features and disjunct distributions in the Old World. Leaf surface and pollen features were investigated in the whole group by SEM and light microscope observations. The section is characterized by simple hairs, a reticulate to undulate cuticle, anomocytic stomata surrounded by a peristomal rim, and trizonocolporate, prolate pollen grains. The characteristics of the indumentum appear constant, while the studied taxa are fairly differentiated with respect to cuticular patterns and dimensions of the stomata, and show slight differences in pollen size and exine surface. This micromorphological evidence, coupled with other phenotypic features, supports the placement of this section at the base of the genus Capparis in the paleotropical area. Considering the striking geographic disjunction and symplesiomorphies of the group, its biogeographical and systematic aspects are also discussed.

The genetic diversity of isolated populations of Cytisus villosus has been studied by means of enzyme polymorphism analysis. Two types of isolated populations were studied: “terrestrial islands” in Sicily, and “true islands” in the Aeolian archipelago. In the populations of “true islands” the number of alleles and the heterozygosity are lower than in “terrestrial islands”. Isolation amongst Sicilian populations seems to be more recent than isolation of the Aeolian populations, and may be attributed to climatic changes which occurred during the Holocene and/or to human activities. The disjunction of the Aeolian populations seems much more recent than the origin of the isles themselves; the colonization of the archipelago is attributed to a single, recent dispersal event not followed by local evolution. In view of the biological structure of the Aeolian populations, C. villosus must be regarded as a locally endangered species.