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1. Mechanisms of Mammalian Selenocysteyl-tRNA Synthesis

Author

Takaharu Mizutani, Hiroshi Kurata, and Kenichiro Yamada

Subjects
Inorganic Chemistry, Biochemistry, Chemistry, Organic Chemistry, TRNA synthesis, medicine, medicine.disease_cause, Escherichia coli, Active enzyme, In vitro
Abstract

The mechnisms of synthesis of mammalian selenocysteyl-tRNA were studied with murine active enzyme fractions. (75Se)Selenocysteyl-tRNA was synthesized in vitro by 500kDa protein from seryl-tRNA and (75Se)HSe-, which should be activated with ATP by 20kDa protein. This and other factors in mamalian systems are compared with those in Escherichia coli.

Published
1992
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