1. Downregulation Of LncRNA PMS2L2 In Endometrial Adenocarcinoma Upon Carboplatin Treatment
- Author
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Yuyang Zhang, Dan Zhang, and Xiuyun Sun
- Subjects
0301 basic medicine ,Endometrial adenocarcinoma ,Chemotherapy ,business.industry ,medicine.medical_treatment ,Endometrial cancer ,Inflammation ,medicine.disease ,Deep sequencing ,Carboplatin ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Downregulation and upregulation ,chemistry ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Viability assay ,medicine.symptom ,business - Abstract
Background LncRNA PMS2L2 plays critical protective roles in chondrocytes during lipopolysaccharide-induced inflammation. Our preliminary deep sequencing revealed the altered expression of PMS2L2 in endometrial adenocarcinoma (EA) during chemotherapy. This observation triggered our interest to explore the functions of PMS2L2 in EA. Methods Levels of PMS2L2 in plasma were measured by qPCR. ROC curve analysis was used for diagnostic analysis. Cell viability was analyzed by cell viability assay. Results We showed that plasma PMS2L2 was downregulated in EA patients compared with healthy controls, and downregulation of PMS2L2 distinguished early-stage EA patients from healthy controls. During carboplatin-based chemotherapy, plasma levels of PMS2L2 were significantly downregulated in endometrial cancer patients. Overexpression of PMS2L2 led to decreased viability of EA cells, while PMS2L2 siRNA silencing led to increased viability of EA cells. Conclusion LncRNA PMS2L2 in endometrial cancer was downregulated during carboplatin treatment and regulates chemosensitivity.
- Published
- 2019