1. Purification of Conjugated Linoleic Acid Isomers through a Process Including Lipase-catalyzed Selective Esterification
- Author
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Shuji Adachi, Toshihiro Nagao, Toshio Iwata, Yuji Shimada, Yoshie Yamauchi-Sato, Koji Nagao, Teruyoshi Yanagita, and Akio Sugihara
- Subjects
Conjugated linoleic acid ,Linoleic acid ,Triacylglycerol lipase ,Fractionation ,Chemical Fractionation ,Applied Microbiology and Biotechnology ,Biochemistry ,Catalysis ,Analytical Chemistry ,Adduct ,Linoleic Acid ,chemistry.chemical_compound ,Organic chemistry ,Lipase ,Molecular Biology ,Candida ,Ethanol ,Chromatography ,Esterification ,integumentary system ,biology ,Organic Chemistry ,Stereoisomerism ,General Medicine ,chemistry ,biology.protein ,Urea ,Indicators and Reagents ,lipids (amino acids, peptides, and proteins) ,Biotechnology - Abstract
A mixture of conjugated linoleic acids (CLAs) was prepared by alkali conjugation of high purity linoleic acid. The preparation contained 45.1 wt% cis-9, trans-11 (c9,t11)-CLA, 46.8 wt% trans-10, cis-12 (t10,c12)-CLA, and 5.3 wt% other CLAs. A process comprising Candida rugosa lipase-catalyzed selective esterification with lauryl alcohol, molecular distillation, and urea adduct fractionation under strict conditions in ethanol was very effective for purification of c9,t11- and t10,c12-CLAs. In particular, the urea adduct fractionation efficiently eliminated CLAs except c9,t11- and t10,c12-isomers. Purification of c9,t11- and t10,c12-CLAs from 1.0 kg of the CLA mixture increased the c9,t11-CLA purity to 93.1% with 34% recovery of the initial content, and increased the t10,c12-CLA purity to 95.3% with 31% recovery.
- Published
- 2003