1. Lab-On-Chip UHF-Dielectrophoretic Cytometer for Colorectal Cancer Stem Cells Sorting
- Author
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Elisa Lambert, Elodie Barthout, Thomas Provent, Remi Manczak, Sofiane Saada, Barbara Bessette, Muriel Mathonnet, Fabrice Lalloué, Claire Dalmay, Arnaud Pothier, RF-ELITE : RF-Electronique Imprimée pour les Télécommunications et l'Energie (XLIM-RFEI), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Contrôle de l’Activation Cellulaire, Progression Tumorale et Résistance thérapeutique (CAPTuR), Institut National de la Santé et de la Recherche Médicale (INSERM)-OmégaHealth (ΩHealth), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Service de Chirurgie digestive, endocrinienne et générale [CHU Limoges], CHU Limoges, PLATINOM: PLATeforme de technologie et d’INstrumentation pour l’Optique et les Microondes - Service commun de l’Université de Limoges (PLATINOM), Region Nouvelle Aquitaine : projet Oncosometrack project No 1R30217, FEDER- CPER PILIM (2015-2020) Etat Region Nouvelle Aquitaine, and European Project: 207249, H2020-EU.1.2.1. - FET Open,737164,SUMCASTEC(2017)
- Subjects
[SPI]Engineering Sciences [physics] ,Radiation ,Biological cell manipulation ,microfluidic biosensors ,[SDV]Life Sciences [q-bio] ,colorectal cancer stem cells ,label-free cell sorting ,Radiology, Nuclear Medicine and imaging ,UHF dielectrophoresis ,Instrumentation - Abstract
International audience; In this article, we introduce a method to exploit ultra-high dielectrophoresis (UHF-DEP) with a microfluidic radio frequency device able to sort biological cells by deflecting the trajectory of the targeted population in a continuous flow. This study highlights the capability of a high frequency lab-on-chip implemented UHF-DEP cytometer to isolate cancer stem cells (CSCs). Actually, CSCs sorting by an efficient, fast and low-cost method remains an issue. Above 20 MHz, the intracellular dielectric properties of cells determine the dielectrophoretic behavior of cells and is then very sensitive to the cell cytoplasm content. The proposed cell sorting principle exploits the combination of the fluidic drag and the repulsive dielectrophoretic forces in order to isolate CSCs hidden within differentiated cancer cells. We previously demonstrated the UHF-DEP behavior of cells, as a new type of electromagnetic biomarkers to discriminate cancer stem cells among a tumor population from colorectal cancer cell line.
- Published
- 2023
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